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Literature summary extracted from

  • Morimoto, S.; Azuma, K.; Oshima, T.; Sakamoto, M.
    Purification and properties of a new enzyme, propioin synthase in baker's yeast which forms propioin from propionaldehyde (1988), J. Ferment. Technol., 66, 7-12.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.35 2-mercaptoethanol stimulation at 3.05 and 6.1 mM Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.35
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.35 (CH3COO)2Pb 44% inhibition at 1.08 mM, complete inhibition at 32 mM Saccharomyces cerevisiae
4.1.2.35 (NH4)2SO4 62% inhibition at 80.6 mM, complete inhibition at 806 mM Saccharomyces cerevisiae
4.1.2.35 AgNO3 5% inhibition at 0.32 mM, complete inhibition at 3.2 mM Saccharomyces cerevisiae
4.1.2.35 Cu2+ 73% inhibition at 0.81 mM, complete inhibition at 8.06 mM Saccharomyces cerevisiae
4.1.2.35 EDTA 5% inhibition at 0.24 mM, 45% inhibition at 9.68 mM Saccharomyces cerevisiae
4.1.2.35 Fe3+ 23% inhibition at 0.16 mM, complete inhibition at 32 mM Saccharomyces cerevisiae
4.1.2.35 Hg2+ 86% inhibition at 1.08 mM, complete inhibition at 10.8 mM Saccharomyces cerevisiae
4.1.2.35 iodoacetic acid 68% inhibition at 16 mM, complete inhibition at 35 mM Saccharomyces cerevisiae
4.1.2.35 Na2SO4 38% inhibition at 0.16 mM, 57% inhibition at 0.86 mM Saccharomyces cerevisiae
4.1.2.35 NH4Cl 21% inhibition at 1.7 mM, complete inhibition at 968 mM Saccharomyces cerevisiae
4.1.2.35 Sn2+ 47% inhibition at 8.69 mM, complete inhibition at 16 mM Saccharomyces cerevisiae
4.1.2.35 Zn2+ 63% inhibition at 1.08 mM, complete inhibition at 16 mM Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.35 Ca2+ 23% stimulation at 0.32 mM Saccharomyces cerevisiae
4.1.2.35 Fe2+ 58% stimulation at 0.08 mM, 145% stimulation at 0.16 mM Saccharomyces cerevisiae
4.1.2.35 Mg2+ 37% stimulation at 0.08 mM, 47% stimulation at 0.16 mM Saccharomyces cerevisiae
4.1.2.35 Mn2+ 55% stimulation at 0.5 mM, 125% stimulation at 1.6 mM Saccharomyces cerevisiae
4.1.2.35 Na+ 12% stimulation at 0.16 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.35 96000
-
 equilibrium centrifugation Saccharomyces cerevisiae
4.1.2.35 100000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.35 propionaldehyde + propionaldehyde Saccharomyces cerevisiae
-
 propioin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.35 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.35
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.35 0.91
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.35 propanal + propanal
-
 Saccharomyces cerevisiae 4-hydroxy-3-hexanone
-
 ?
4.1.2.35 propionaldehyde + propionaldehyde
-
 Saccharomyces cerevisiae propioin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.35 37
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.35 30 45 5 min, pH 6.8, about 90% of maximum activity Saccharomyces cerevisiae
4.1.2.35 50
-
 5 min, pH 6.8, about 40% of maximum activity Saccharomyces cerevisiae
4.1.2.35 55
-
 5 min, pH 6.8, about 5% of maximum activity Saccharomyces cerevisiae
4.1.2.35 60
-
 5 min, pH 6.8, complete inactivation Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.35 6.8 7
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.35 6 10 18% activity t pH 6, 30% activity at pH 10 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.35 3
-
 complete inactivation Saccharomyces cerevisiae
4.1.2.35 7 8 most stable Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.35 thiamine diphosphate 10% stimulation at 0.16 mM, 65% stimulation at 0.4 mM Saccharomyces cerevisiae