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Literature summary extracted from

  • Prestwich, G.D.; Angelastro, M.; de Palma, A.; Perino, M.A.
    Fucosterol epoxide lyase of insects: synthesis of labeled substrates and development of a partition assay (1985), Anal. Biochem., 151, 315-326.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.33 2-mercaptoethanol 10 mM: 103% of maximal activity, slight activation Manduca sexta
4.1.2.33 NADH 10 mM: 139% of maximal activity, activation Manduca sexta
4.1.2.33 NADP+ 10 mM: 109% of maximal activity, activation Manduca sexta
4.1.2.33 NADPH 10 mM: 106% of maximal activity, slight activation Manduca sexta
4.1.2.33 p-hydroxymercuribenzoate 1 mM: 102% of maximal activity, slight activation Manduca sexta

General Stability

EC Number General Stability Organism
4.1.2.33 no activity is lost in a single freeze-thaw cycle Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.33 Ca2+ 10 mM: 16% of maximal activity Manduca sexta
4.1.2.33 chymotrypsin 0.05 mg/ml: 57% of maximal activity Manduca sexta
4.1.2.33 D-glucose 200 mM: 42% of maximal activity Manduca sexta
4.1.2.33 EDTA 10 mM: 66% of maximal activity Manduca sexta
4.1.2.33 Fe2+ 10 mM: 18% of maximal activity Manduca sexta
4.1.2.33 Fe3+ 10 mM: 26% of maximal activity Manduca sexta
4.1.2.33 glutathione oxidized, 10 mM: 58% of maximal activity, reduced: 10 mM, 89% of maximal activity Manduca sexta
4.1.2.33 imidazole 10 mM: 99% of maximal activity Manduca sexta
4.1.2.33 K+ 10 mM: 71% of maximal activity Manduca sexta
4.1.2.33 Lubrol Px 1% (w/v): complete inhibition Manduca sexta
4.1.2.33 Mg2+ 10 mM: 75% of maximal activity Manduca sexta
4.1.2.33 N-ethylmaleimide 10 mM: 77% of maximal activity Manduca sexta
4.1.2.33 NAD+ 10 mM: 95% of maximal activity Manduca sexta
4.1.2.33 phenylmethylsulfonyl fluoride 10 mM: 63% of maximal activity Manduca sexta
4.1.2.33 Staphylococcus aureus V8 protease 0.05 mg/ml: 20% of maximal activity Manduca sexta
4.1.2.33 sucrose 200 mM: 73% of maximal activity Manduca sexta
4.1.2.33 Triton X-100 1% (w/v): 12% of maximal activity Manduca sexta
4.1.2.33 Zn2+ 10 mM: 9% of maximal activity Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.33 0.0007
-
(24RS,28RS)-fucosterol epoxide 37°C Manduca sexta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.33 (24R,241R)-fucosterol epoxide Manduca sexta
-
desmosterol + acetaldehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.33 Manduca sexta
-
tobacco hornworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.33 larval tobacco hornworm, partial Manduca sexta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.33 fat body
-
Manduca sexta
-
4.1.2.33 midgut
-
Manduca sexta
-

Storage Stability

EC Number Storage Stability Organism
4.1.2.33 -80°C, no activity is lost in a single freeze-cycle Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.33 (24R,241R)-fucosterol epoxide
-
Manduca sexta desmosterol + acetaldehyde
-
?
4.1.2.33 (24R,241R)-fucosterol epoxide best substrate Manduca sexta desmosterol + acetaldehyde demonstrated in crude extract ?
4.1.2.33 (24RS,28RS)-fucosterol epoxide
-
Manduca sexta ?
-
?
4.1.2.33 (24S,28S)-fucosterol epoxide
-
Manduca sexta ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.33 37
-
-
Manduca sexta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.33 27
-
broad range of activity Manduca sexta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.33 7.4
-
-
Manduca sexta