Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furuyoshi, S.; Nawa, Y.; Kawabata, N.; Tanaka, H.; Soda, K.
    Purification and characterization of a new NAD(+)-dependent enzyme, L-tartrate decarboxylase, from Pseudomonas sp. group Ve-2 (1991), J. Biochem., 110, 520-525.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.73 D-glycerate product inhibition, 10 mM, 65% inhibition Pseudomonas sp.
4.1.1.73 D-malate 10 mM, 83% inhibition Pseudomonas sp.
4.1.1.73 EDTA 5 mM, at 30°C, complete inactivation Pseudomonas sp.
4.1.1.73 L-Glycerate 10 mM, 63% inhibition Pseudomonas sp.
4.1.1.73 L-malate 10 mM, 29% inhibition Pseudomonas sp.
4.1.1.73 meso-tartrate 10 mM, 14% inhibition Pseudomonas sp.
4.1.1.73 succinate 10 mM, 28% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.73 1.1
-
 NAD+ pH 7.5, 30°C Pseudomonas sp.
4.1.1.73 7
-
 L-Tartrate pH 7.5, 30°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.73 Co2+ activation Pseudomonas sp.
4.1.1.73 Cu2+ slight activation Pseudomonas sp.
4.1.1.73 K+ activation, above 40 mM Pseudomonas sp.
4.1.1.73 Mg2+ activation Pseudomonas sp.
4.1.1.73 Mg2+ above 0.2 mM Pseudomonas sp.
4.1.1.73 Mn2+ activation Pseudomonas sp.
4.1.1.73 additional information requirement of a divalent and a monovalent cation, not activated by Ca2+, Fe2+, Zn2+, Li+ or Na+ Pseudomonas sp.
4.1.1.73 NH4+ slight activation Pseudomonas sp.
4.1.1.73 Ni2+ slight activation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.73 40000
-
 4 * 40000, SDS-PAGE Pseudomonas sp.
4.1.1.73 148000
-
 HPLC gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.73 L-tartrate Pseudomonas sp. inducible by L-tartrate D-glycerate + CO2
-
 ?
4.1.1.73 L-tartrate Pseudomonas sp. 5D1A inducible by L-tartrate D-glycerate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.73 Pseudomonas sp.
-
 soil isolate
-
4.1.1.73 Pseudomonas sp.
-
 group Ve-2
-
4.1.1.73 Pseudomonas sp. 5D1A
-
 group Ve-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.73 about 11fold Pseudomonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.73 (R,R)-tartrate = D-glycerate + CO2 reduction proceeds concomitantly with decarboxylation, but at a much lower rate Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.73 4.8
-
 pH 7.5, 30°C Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.1.1.73 -20°C, 50% glycerol, 3 months, stable, without glycerol, 1 month, 40% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.73 D-malate + NAD+
-
 Pseudomonas sp. pyruvate + CO2 + NADH
-
 ?
4.1.1.73 D-malate + NAD+
-
 Pseudomonas sp. 5D1A pyruvate + CO2 + NADH
-
 ?
4.1.1.73 L-tartrate best substrate Pseudomonas sp. D-glycerate + CO2
-
 ?
4.1.1.73 L-tartrate inducible by L-tartrate Pseudomonas sp. D-glycerate + CO2
-
 ?
4.1.1.73 L-tartrate best substrate Pseudomonas sp. 5D1A D-glycerate + CO2
-
 ?
4.1.1.73 L-tartrate inducible by L-tartrate Pseudomonas sp. 5D1A D-glycerate + CO2
-
 ?
4.1.1.73 meso-tartrate
-
 Pseudomonas sp. D-glycerate + CO2
-
 ?
4.1.1.73 additional information not: dihydroxyfumarate Pseudomonas sp. ?
-
 ?
4.1.1.73 additional information enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity Pseudomonas sp. ?
-
 ?
4.1.1.73 additional information not: dihydroxyfumarate Pseudomonas sp. 5D1A ?
-
 ?
4.1.1.73 additional information enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity Pseudomonas sp. 5D1A ?
-
 ?
4.1.1.73 oxaloacetate + NAD+
-
 Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.73 homotetramer 4 * 40000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.1.73 More enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.73 30
-
 assay at Pseudomonas sp.
4.1.1.73 40
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.73 50
-
 30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, stable Pseudomonas sp.
4.1.1.73 60
-
 30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 5% loss of activity, inactivation at pH 6.5 and 9.5 Pseudomonas sp.
4.1.1.73 65
-
 30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 29% loss of activity Pseudomonas sp.
4.1.1.73 70
-
 30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, inactivation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.73 7 8
-
 Pseudomonas sp.
4.1.1.73 7.5
-
 assay at Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.73 5.5 9 half-maximal activity at pH 5.5 and 9 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.73 6.5
-
 30 min at 60°C, inactivation Pseudomonas sp.
4.1.1.73 7
-
 30 min at 60°C, 23% loss of activity Pseudomonas sp.
4.1.1.73 7.5
-
 30 min at 60°C, in 20 mM Tris-HCl buffer, 0.2 mM MgCl2, 5% loss of activity Pseudomonas sp.
4.1.1.73 8
-
 30 min at 60°C, 13% loss of activity Pseudomonas sp.
4.1.1.73 9
-
 30 min at 60°C, 57% loss of activity Pseudomonas sp.
4.1.1.73 9.5
-
 30 min at 60°C, inactivation Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.73 NAD+ requirement Pseudomonas sp.
4.1.1.73 NAD+ decarboxylation is not stoichiometrically related with NAD+, NAD+-reduction at 1.1% the rate of L-tartrate decarboxylation Pseudomonas sp.
4.1.1.73 NADP+ requirement, less effective than NAD+ Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.73 Pseudomonas sp. isoelectric focusing
-
 4.9