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Literature summary extracted from
- The biotin-dependent sodium ion pump glutaconyl-CoA decarboxylase from Fusobacterium nucleatum (subsp. nucleatum) (1990), Arch. Microbiol., 154, 362-369.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.4.5 | biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus |  |
| 7.2.4.5 | biotin | biotinyl enzyme | Fusobacterium nucleatum | |
| 7.2.4.5 | biotin | biotinyl enzyme | [Clostridium] symbiosum | |
| 7.2.4.5 | biotin | biotinyl enzyme | Acidaminococcus fermentans | |
| 7.2.4.5 | Phospholipid | no stimulation | [Clostridium] symbiosum | |
| 7.2.4.5 | Phospholipid | no stimulation | Acidaminococcus fermentans | |
| 7.2.4.5 | Phospholipid | stimulates enzyme | Fusobacterium nucleatum | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.2.4.5 | requires Na+ for stability | Fusobacterium nucleatum |
| 7.2.4.5 | unstable in the presence of buffers containing potassium | Fusobacterium nucleatum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.4.5 | membrane | - |
Peptoniphilus asaccharolyticus | 16020 | - |
| 7.2.4.5 | membrane | - |
Fusobacterium nucleatum | 16020 | - |
| 7.2.4.5 | membrane | - |
[Clostridium] symbiosum | 16020 | - |
| 7.2.4.5 | membrane | - |
Acidaminococcus fermentans | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.4.5 | Na+ | required | Peptoniphilus asaccharolyticus | |
| 7.2.4.5 | Na+ | required | Fusobacterium nucleatum | |
| 7.2.4.5 | Na+ | required | [Clostridium] symbiosum | |
| 7.2.4.5 | Na+ | required | Acidaminococcus fermentans | |
| 7.2.4.5 | Na+ | Km: 1 mM | Acidaminococcus fermentans | |
| 7.2.4.5 | Na+ | Km: 3 mM | Fusobacterium nucleatum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.4.5 | 16000 | - |
x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE | Fusobacterium nucleatum |
| 7.2.4.5 | 19000 | - |
x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE | Fusobacterium nucleatum |
| 7.2.4.5 | 33000 | - |
x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE | Fusobacterium nucleatum |
| 7.2.4.5 | 65000 | - |
x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE | Fusobacterium nucleatum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | Peptoniphilus asaccharolyticus | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | Fusobacterium nucleatum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | [Clostridium] symbiosum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | Acidaminococcus fermentans | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 7.2.4.5 | 1-butanol | concentration 2%, 50% loss of activity after 2 min | Acidaminococcus fermentans |
| 7.2.4.5 | 1-butanol | concentration 1%, 50% loss of activity after 1 min in presence of 200 mM KCl, 50% loss of activity after 3 min in presence of 200 mM NaCl | Fusobacterium nucleatum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.4.5 | Acidaminococcus fermentans | - |
ATCC 25085 | - |
| 7.2.4.5 | Fusobacterium nucleatum | - |
subsp. nucleatum | - |
| 7.2.4.5 | Peptoniphilus asaccharolyticus | - |
- |
- |
| 7.2.4.5 | [Clostridium] symbiosum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.4.5 | - |
Peptoniphilus asaccharolyticus |
| 7.2.4.5 | - |
[Clostridium] symbiosum |
| 7.2.4.5 | - |
Acidaminococcus fermentans |
| 7.2.4.5 | subsp. nucleatum | Fusobacterium nucleatum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.2.4.5 | additional information | - |
- |
Peptoniphilus asaccharolyticus |
| 7.2.4.5 | additional information | - |
- |
Fusobacterium nucleatum |
| 7.2.4.5 | additional information | - |
- |
[Clostridium] symbiosum |
| 7.2.4.5 | additional information | - |
- |
Acidaminococcus fermentans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.2.4.5 | -80°C, 24 h, 100 mM Na+, stable | Fusobacterium nucleatum |
| 7.2.4.5 | 4°C, 24 h, 100 mM Na+, 50% loss of activity | Fusobacterium nucleatum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.4.5 | 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 7.2.4.5 | glutaconyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.4.5 | ? | - |
Acidaminococcus fermentans |
| 7.2.4.5 | ? | x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE | Fusobacterium nucleatum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.2.4.5 | 37 | - |
200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min | Peptoniphilus asaccharolyticus |
| 7.2.4.5 | 37 | - |
200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min | Fusobacterium nucleatum |
| 7.2.4.5 | 37 | - |
200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min | [Clostridium] symbiosum |
| 7.2.4.5 | 37 | - |
200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min | Acidaminococcus fermentans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.4.5 | biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus | |
| 7.2.4.5 | biotin | biotinyl enzyme | Fusobacterium nucleatum | |
| 7.2.4.5 | biotin | biotinyl enzyme | [Clostridium] symbiosum | |
| 7.2.4.5 | biotin | biotinyl enzyme | Acidaminococcus fermentans | |
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