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Literature summary extracted from

  • Wohlfarth, G.; Buckel, P.; Buckel, W.
    On the structure and function of glutaconyl-CoA, a biotin dependent sodium pump from anaerobic bacteria (1986), Prog. Biotechnol., 2, 111-116.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.4.5 biotin biotinyl enzyme Peptoniphilus asaccharolyticus
7.2.4.5 biotin biotinyl enzyme Acidaminococcus fermentans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.5
-
 Acidaminococcus fermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.5 membrane
-
 Peptoniphilus asaccharolyticus 16020
-
7.2.4.5 membrane
-
 Acidaminococcus fermentans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.4.5 Na+ required Peptoniphilus asaccharolyticus
7.2.4.5 Na+ required Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.4.5 30000
-
 x * 120000, alpha, biotin carrier + x * 60000, beta, transferase + x * 30000, gamma, sodium-dependent carboxybiotin decarboxylase, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 60000
-
 x * 120000, alpha, biotin carrier + x * 60000, beta, transferase + x * 30000, gamma, sodium-dependent carboxybiotin decarboxylase, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 120000
-
 x * 120000, alpha, biotin carrier + x * 60000, beta, transferase + x * 30000, gamma, sodium-dependent carboxybiotin decarboxylase, SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.5 glutaconyl-CoA + Na+[side 1] Peptoniphilus asaccharolyticus
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1] Acidaminococcus fermentans
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.5 Acidaminococcus fermentans
-
 ATCC 25085
-
7.2.4.5 Peptoniphilus asaccharolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.5 ? x * 120000, alpha, biotin carrier + x * 60000, beta, transferase + x * 30000, gamma, sodium-dependent carboxybiotin decarboxylase, SDS-PAGE Acidaminococcus fermentans

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.5 biotin biotinyl enzyme Peptoniphilus asaccharolyticus
7.2.4.5 biotin biotinyl enzyme Acidaminococcus fermentans