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Literature summary extracted from

  • Buckel, W.; Semmler, R.
    Purification, characterisation and reconstitution of glutaconyl-CoA decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria (1983), Eur. J. Biochem., 136, 427-434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.4.5 biotin biotinyl enzyme Peptoniphilus asaccharolyticus
7.2.4.5 biotin biotinyl enzyme Clostridium tetanomorphum
7.2.4.5 biotin biotinyl enzyme [Clostridium] symbiosum
7.2.4.5 biotin biotinyl enzyme Acidaminococcus fermentans
7.2.4.5 Triton X-100 activates Peptoniphilus asaccharolyticus
7.2.4.5 Triton X-100 activates Clostridium tetanomorphum
7.2.4.5 Triton X-100 activates [Clostridium] symbiosum
7.2.4.5 Triton X-100 activates Acidaminococcus fermentans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.5
-
 Peptoniphilus asaccharolyticus
7.2.4.5
-
 Clostridium tetanomorphum
7.2.4.5
-
 [Clostridium] symbiosum
7.2.4.5
-
 Acidaminococcus fermentans

General Stability

EC Number General Stability Organism
7.2.4.5 sodium ions specifically protect the gamma chain from tryptic digestion Peptoniphilus asaccharolyticus
7.2.4.5 sodium ions specifically protect the gamma chain from tryptic digestion Clostridium tetanomorphum
7.2.4.5 sodium ions specifically protect the gamma chain from tryptic digestion [Clostridium] symbiosum
7.2.4.5 sodium ions specifically protect the gamma chain from tryptic digestion Acidaminococcus fermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.4.5 Avidin
-
 Acidaminococcus fermentans
7.2.4.5 Avidin
-
 [Clostridium] symbiosum
7.2.4.5 Avidin
-
 Clostridium tetanomorphum
7.2.4.5 Avidin
-
 Peptoniphilus asaccharolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.5 membrane integral membrane protein Peptoniphilus asaccharolyticus 16020
-
7.2.4.5 membrane integral membrane protein Clostridium tetanomorphum 16020
-
7.2.4.5 membrane integral membrane protein [Clostridium] symbiosum 16020
-
7.2.4.5 membrane integral membrane protein Acidaminococcus fermentans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.4.5 35000
-
 1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 60000
-
 1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.5 glutaconyl-CoA + Na+[side 1] Peptoniphilus asaccharolyticus
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1] Clostridium tetanomorphum
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1] [Clostridium] symbiosum
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1] Acidaminococcus fermentans
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.5 Acidaminococcus fermentans
-
 ATCC 25085
-
7.2.4.5 Clostridium tetanomorphum
-
-
-
7.2.4.5 Peptoniphilus asaccharolyticus
-
-
-
7.2.4.5 [Clostridium] symbiosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.4.5
-
 Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Clostridium tetanomorphum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 [Clostridium] symbiosum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Clostridium tetanomorphum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 [Clostridium] symbiosum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.5 ? 1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE Acidaminococcus fermentans

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.5 biotin biotinyl enzyme Peptoniphilus asaccharolyticus
7.2.4.5 biotin biotinyl enzyme Clostridium tetanomorphum
7.2.4.5 biotin biotinyl enzyme [Clostridium] symbiosum
7.2.4.5 biotin biotinyl enzyme Acidaminococcus fermentans