Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kurihara, T.; Liu, J.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K.
    Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues (1995), J. Biochem., 117, 1317-1322.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.2 additional information mutants of all the 36 highly conserved charged and polar amino acid residues of enzyme, D10, D180, R41, K151, S118, S175, T14, Y157, and N177 play indispensable roles in the catalysis Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.37
-
L-2-chloropropionate comparison with mutant enzymes Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Burkholderia cepacia
-
-
-
3.8.1.2 Burkholderia cepacia MBA4
-
-
-
3.8.1.2 Moraxella sp.
-
-
-
3.8.1.2 Moraxella sp. B
-
-
-
3.8.1.2 Pseudomonas putida
-
AJ1
-
3.8.1.2 Pseudomonas putida
-
No. 109
-
3.8.1.2 Pseudomonas putida AJ1
-
AJ1
-
3.8.1.2 Pseudomonas putida No. 109
-
No. 109
-
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.2 Xanthobacter autotrophicus
-
GJ10
-
3.8.1.2 Xanthobacter autotrophicus GJ10
-
GJ10
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 additional information
-
specific activities of diverse mutants Pseudomonas sp.
3.8.1.2 100
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
Pseudomonas sp. (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Pseudomonas putida (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Moraxella sp. (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Burkholderia cepacia (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Xanthobacter autotrophicus (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Pseudomonas putida AJ1 (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Burkholderia cepacia MBA4 (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Xanthobacter autotrophicus GJ10 (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Pseudomonas putida No. 109 (R)-2-hydroxyacid + halide
-
?
3.8.1.2 (S)-2-haloacid + H2O
-
Moraxella sp. B (R)-2-hydroxyacid + halide
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Pseudomonas sp. D-lactate + HCl
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Pseudomonas putida D-lactate + HCl
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Xanthobacter autotrophicus D-lactate + HCl
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Pseudomonas putida AJ1 D-lactate + HCl
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Xanthobacter autotrophicus GJ10 D-lactate + HCl
-
?
3.8.1.2 L-2-chloropropionate + H2O
-
Pseudomonas putida No. 109 D-lactate + HCl
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.2 47
-
L-2-chloropropionate comparison with mutant enzymes Pseudomonas sp.