EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.8.1.2 | additional information | mutants of all the 36 highly conserved charged and polar amino acid residues of enzyme, D10, D180, R41, K151, S118, S175, T14, Y157, and N177 play indispensable roles in the catalysis | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.2 | 0.37 | - |
L-2-chloropropionate | comparison with mutant enzymes | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.2 | Burkholderia cepacia | - |
- |
- |
3.8.1.2 | Burkholderia cepacia MBA4 | - |
- |
- |
3.8.1.2 | Moraxella sp. | - |
- |
- |
3.8.1.2 | Moraxella sp. B | - |
- |
- |
3.8.1.2 | Pseudomonas putida | - |
AJ1 | - |
3.8.1.2 | Pseudomonas putida | - |
No. 109 | - |
3.8.1.2 | Pseudomonas putida AJ1 | - |
AJ1 | - |
3.8.1.2 | Pseudomonas putida No. 109 | - |
No. 109 | - |
3.8.1.2 | Pseudomonas sp. | - |
- |
- |
3.8.1.2 | Xanthobacter autotrophicus | - |
GJ10 | - |
3.8.1.2 | Xanthobacter autotrophicus GJ10 | - |
GJ10 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | additional information | - |
specific activities of diverse mutants | Pseudomonas sp. |
3.8.1.2 | 100 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.2 | (S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Pseudomonas putida | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Moraxella sp. | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Burkholderia cepacia | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Xanthobacter autotrophicus | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Pseudomonas putida AJ1 | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Burkholderia cepacia MBA4 | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Xanthobacter autotrophicus GJ10 | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Pseudomonas putida No. 109 | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | (S)-2-haloacid + H2O | - |
Moraxella sp. B | (R)-2-hydroxyacid + halide | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Pseudomonas sp. | D-lactate + HCl | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Pseudomonas putida | D-lactate + HCl | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Xanthobacter autotrophicus | D-lactate + HCl | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Pseudomonas putida AJ1 | D-lactate + HCl | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Xanthobacter autotrophicus GJ10 | D-lactate + HCl | - |
? | |
3.8.1.2 | L-2-chloropropionate + H2O | - |
Pseudomonas putida No. 109 | D-lactate + HCl | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.2 | 47 | - |
L-2-chloropropionate | comparison with mutant enzymes | Pseudomonas sp. |