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Literature summary extracted from

  • Liu, J.Q.; Kurihara, T.; Miyagi, M.; Esaki, N.; Soda, K.
    Reaction mechanism of L-2-haloacid dehalogenase of Pseudomonas sp. YL. Identification of Asp10 as the active site nucleophile by 18O incorporation experiments (1995), J. Biol. Chem., 270, 18309-18312.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.2 additional information mutant enzyme is constructed by introducing three lysyl residues at positions 11, 176 and 185 of enzyme by site-directed mutagenesis Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 MnSO4
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp. 113
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.2 Pseudomonas sp. 113
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.8.1.2 (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product Pseudomonas sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 DL-2-bromo-n-butyrate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.2 DL-2-bromo-n-butyrate + H2O
-
 Pseudomonas sp. 113 ?
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. 113 D-lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. 113 D-2-hydroxyalkanoic acid + halide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 45
-
-
 Pseudomonas sp.