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Literature summary extracted from

  • Hasan, A.K.M.Q.; Takada, H.; Koshikawa, H.; Liu, J.Q.; Kurihara, T.; Esaki, N.; Soda, K.
    Two kinds of 2-halo acid dehalogeases fromm Pseudomonas sp. YL induced by 2-chloroacrylates and 2-chloropropionate (1994), Biosci. Biotechnol. Biochem., 58, 1599-1602.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.10 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 N-ethylmaleimide
-
 Pseudomonas sp.
3.8.1.2 p-mercuric chlorobenzoate
-
 Pseudomonas sp.
3.8.1.2 ZnSO4
-
 Pseudomonas sp.
3.8.1.10 N-ethylmaleimide
-
 Pseudomonas sp.
3.8.1.10 p-chloromercuribenzoate
-
 Pseudomonas sp.
3.8.1.10 Zn2+ ZnSO4 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O Pseudomonas sp.
-
 glycolate + HCl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.10 Pseudomonas sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.8.1.2 culture condition:2-chloropropionate-grown cell
-
 Pseudomonas sp.
-
3.8.1.10 culture condition:2-chloroacrylate-grown cell
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O enzyme of 2-chloropropionate grown cells Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2,2-dichloropropionate + H2O
-
 Pseudomonas sp. propionate + HCl
-
 ?
3.8.1.2 dichloroacetate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.2 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.10 (R)-2-haloacid + H2O enzyme of 2-chloroacrylate grown cells Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O enzyme of 2-chloroacrylate grown cells Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 2,2-dichloropropionate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.10 2,3-dichloropropionate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.10 2-chloro-3-hydroxypropionate + H2O
-
 Pseudomonas sp. glycerate + HCl
-
 ?
3.8.1.10 2-chloro-n-butyrate + H2O
-
 Pseudomonas sp. 2-hydroxybutyrate + HCl
-
 ?
3.8.1.10 D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
3.8.1.10 dichloroacetate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.10 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. DL-lactate + HCl
-
 ?
3.8.1.10 L-2-monochloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.10 50
-
 15 min 50% loss of activity for D-2-chloropropionic acid and 40% loss of activity for L-2-chloropropionic acid Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 9.5
-
-
 Pseudomonas sp.
3.8.1.10 10
-
-
 Pseudomonas sp.