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Literature summary extracted from

  • Nardi-Dei, V.; Kurihara, T.; Park, C.; Esaki, N.; Soda, K.
    Bacterial DL-2-haloacid dehalogenase from Pseudomonas sp. strain 113: gene cloning and structural comparison with D- and L-2-haloacid dehalogenases (1997), J. Bacteriol., 179, 4232-4238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.10 expression in Escherichia coli, no significant sequence similarity between enzyme and L-2-haloacid dehalogenase is found, however, enzyme has significant sequence similarity with D-2-haloacid dehalogenase fom Pseudomonas putide AJ1 Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.10 additional information each of the 26 residues with charged and polar side chains, which are conserved between enzyme and D-2-haloacid dehalogenase, is mutated, Thr65, Glu69 and Asp194 are found to be essential Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.10 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide complete inactivation Pseudomonas sp.
3.8.1.10 D-2-chloropropionate inhibits dehalogenation of L-2-chloropropionate Pseudomonas sp.
3.8.1.10 diethyldicarbonate significant inactivation Pseudomonas sp.
3.8.1.10 L-2-chloropropionate inhibits dehalogenation of D-2-chloropropionate Pseudomonas sp.
3.8.1.10 N-bromosuccinimide significant inactivation Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.10 0.9
-
 L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.10 4.2
-
 D-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.10 4.8
-
 Monochloroacetate pH 9.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.10 34242
-
 2 * 34242, calculated from amino acid sequence Pseudomonas sp.
3.8.1.10 68000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.10 (R)-2-haloacid + H2O Pseudomonas sp.
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O Pseudomonas sp. 113
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O Pseudomonas sp.
-
 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O Pseudomonas sp. 113
-
 (R)-2-hydroxyacid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.10 Achromobacter xylosoxidans Q59168
-
-
3.8.1.10 Pseudomonas putida
-
-
-
3.8.1.10 Pseudomonas putida AJ1
-
-
-
3.8.1.10 Pseudomonas sp. O06652
-
-
3.8.1.10 Pseudomonas sp. 113 O06652
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.10 recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas putida (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas putida AJ1 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas putida (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas putida AJ1 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 D-2-chloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
3.8.1.10 D-2-chloropropionate + H2O
-
 Pseudomonas sp. 113 L-lactate + HCl
-
 ?
3.8.1.10 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.10 L-2-chloropropionate + H2O
-
 Pseudomonas sp. 113 D-lactate + HCl
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.10 dimer 2 * 34242, calculated from amino acid sequence Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.8.1.10 5.2
-
 D-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.10 6.5
-
 L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.