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Literature summary extracted from

  • Hata, S.; Sato, T.; Sorimachi, H.; Ishiura, S.; Suzuki, K.
    A simple purification and fluorescent assay method of the poliovirus 3C protease searching for specific inhibitors (2000), J. Virol. Methods, 84, 117-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.28 a cDNA fragment containing the 3C protease coding region is inserted into pET22b vector and expressed in Escherichia coli Enterovirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.28 2,2'-dipyridyl disulfide 0.4 mM, complete inhibition Enterovirus C
3.4.22.28 Aprotinin 0.1 mM, 64% inhibition Enterovirus C
3.4.22.28 calpastatin 0.8 mM, 30% inhibition Enterovirus C
3.4.22.28 chymostatin 0.1 mM, 54% inhibition Enterovirus C
3.4.22.28 iodoacetic acid 0.4 mM, complete inhibition Enterovirus C
3.4.22.28 PMSF 1.7 mM, 32% inhibition Enterovirus C
3.4.22.28 TLCK 0.2 mM, 51% inhibition Enterovirus C
3.4.22.28 Zn2+ 2.0 mM ZnCl2, complete inhibition Enterovirus C

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.28 24000
-
 x * 24000, SDS-PAGE Enterovirus C

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.28 poliovirus polyprotein + H2O Enterovirus C the polyprotein is cleaved into mature proteins predominantly by the viral 3C protease hydrolyzed poliovirus polyprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.28 Enterovirus C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.28
-
 Enterovirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.28 7-methoxycoumarin-4-yl-MEALFQGPLQY-2,4-dinitrophenyl amide + H2O
-
 Enterovirus C ?
-
 ?
3.4.22.28 poliovirus polyprotein + H2O the polyprotein is cleaved into mature proteins predominantly by the viral 3C protease Enterovirus C hydrolyzed poliovirus polyprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.28 ? x * 24000, SDS-PAGE Enterovirus C