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Literature summary extracted from

  • Petanceska, S.; Devi, L.
    Sequence analysis, tissue distribution, and expression of rat cathepsin S (1992), J. Biol. Chem., 267, 26038-26043.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.27 expression as a glutathione S-transferase-fusion protein Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.27 cystatin 0.5 mg/ml, 82% inhibition Rattus norvegicus
3.4.22.27 E-64 0.005 mM, 96% inhibition Rattus norvegicus
3.4.22.27 iodoacetic acid 1 mM, 92% inhibition Rattus norvegicus
3.4.22.27 leupeptin 1 mM, complete inhibition Rattus norvegicus
3.4.22.27 NEM 1 mM, complete inhibition Rattus norvegicus
3.4.22.27 p-chloromercuriphenylsulfonic acid 1 mM, 96% inhibition Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.27 additional information Rattus norvegicus cathepsin S is possibly involved in thyroid hormone biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.27 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.27 proteolytic modification the cDNA encodes a 330-residue protein with a 17-amino acid putative signal sequence, 95-residue ürpregion, and 216-residue mature protein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.27 brain
-
 Rattus norvegicus
-
3.4.22.27 heart low activity Rattus norvegicus
-
3.4.22.27 ileum
-
 Rattus norvegicus
-
3.4.22.27 jejunum low activity Rattus norvegicus
-
3.4.22.27 kidney low activity Rattus norvegicus
-
3.4.22.27 liver low activity Rattus norvegicus
-
3.4.22.27 lung low activity Rattus norvegicus
-
3.4.22.27 ovary
-
 Rattus norvegicus
-
3.4.22.27 spleen
-
 Rattus norvegicus
-
3.4.22.27 thyroid gland
-
 Rattus norvegicus
-
3.4.22.27 uterus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.27 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.27 additional information cathepsin S is possibly involved in thyroid hormone biosynthesis Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.27 37
-
 15 min, retention of more than 80% enzyme activity at pH 7.0, about 40% retention at pH 4.7 and about 50% retention at pH 8.5 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.27 5.5 8.5 pH 5.5: about 45% of maximal activity, pH 8.5: about 50% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.27 4.7
-
 37°C, about 40% retention of activity after 15 min Rattus norvegicus
3.4.22.27 7
-
 37°C, more than 80% retention of activity after 15 min Rattus norvegicus
3.4.22.27 8.5
-
 37°C, about 50% retention of activity after 15 min Rattus norvegicus