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Literature summary extracted from

  • Yamashita, M.; Konagaya, S.
    Purification and characterization of cathepsin I from the white muscle of chum salmon, Oncorhynchus keta (1990), Comp. Biochem. Physiol. B, 96, 247-252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 2-mercaptoethanol enhances activity Oncorhynchus keta
3.4.22.15 dithiothreitol enhances activity Oncorhynchus keta
3.4.22.15 EDTA enhances activity Oncorhynchus keta
3.4.22.15 thiol-reducing agent required Oncorhynchus keta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 antipain
-
 Oncorhynchus keta
3.4.22.15 chymostatin
-
 Oncorhynchus keta
3.4.22.15 Cys
-
 Oncorhynchus keta
3.4.22.15 Hg2+ HgCl2 Oncorhynchus keta
3.4.22.15 iodoacetic acid
-
 Oncorhynchus keta
3.4.22.15 leupeptin
-
 Oncorhynchus keta
3.4.22.15 NEM
-
 Oncorhynchus keta
3.4.22.15 PCMB
-
 Oncorhynchus keta
3.4.22.15 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Oncorhynchus keta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.15 0.00168
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Oncorhynchus keta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 30000
-
 gel filtration Oncorhynchus keta

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Oncorhynchus keta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Oncorhynchus keta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 muscle white muscle Oncorhynchus keta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 additional information
-
-
 Oncorhynchus keta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 azocasein + H2O
-
 Oncorhynchus keta ?
-
 ?
3.4.22.15 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Oncorhynchus keta ?
-
 ?
3.4.22.15 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Oncorhynchus keta ?
-
 ?
3.4.22.15 Hemoglobin + H2O
-
 Oncorhynchus keta ?
-
 ?
3.4.22.15 Insulin B-chain + H2O main cleavage sites: Glu13-Ala14 and Tyr26-Thr27, minor cleavage sites: Val2-Asn3, Asn3-Gln4, Cys7-Glu8, Tyr16-Leu17, Leu17-Val18 Oncorhynchus keta ?
-
 ?
3.4.22.15 additional information the enzyme has a preference for hydrophobic amino acids in P2 and P3 residues Oncorhynchus keta ?
-
 ?
3.4.22.15 serum albumin + H2O
-
 Oncorhynchus keta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 monomer 1 * 30000, SDS-PAGE Oncorhynchus keta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.15 15.8
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Oncorhynchus keta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 5.6
-
 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Oncorhynchus keta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 4.2 6.7 pH 4.2: about 50% of maximal activity, pH 6.7: about 35% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Oncorhynchus keta