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Literature summary extracted from

  • V÷lkel, H.; Kurz, U.; Linder, J.; Klumpp, S.; Gnau, V.; Jung, G.; Schultz, J.E.
    Cathepsin L is an intracellular and extracellular protease in Paramecium tetraurelia. Purification, cloning, sequencing and specific inhibition by its expressed propeptide (1996), Eur. J. Biochem., 238, 198-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.15 the cDNA codes for a preproregion of 108 amino acids. The putative proregion of 86 amino acids which contains the characteristic conserved ERFNIN motif, is fused with a His6 tag, expressed in Escherichia coli Paramecium tetraurelia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 CuCl2
-
 Paramecium tetraurelia
3.4.22.15 cystatin
-
 Paramecium tetraurelia
3.4.22.15 leupeptin
-
 Paramecium tetraurelia
3.4.22.15 NEM
-
 Paramecium tetraurelia
3.4.22.15 p-hydroxymercuribenzoate
-
 Paramecium tetraurelia
3.4.22.15 propeptide
-
 Paramecium tetraurelia
3.4.22.15 tosyl-Lys-CH2Cl
-
 Paramecium tetraurelia
3.4.22.15 ZnCl2
-
 Paramecium tetraurelia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 extracellular and intracellular Paramecium tetraurelia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 22161
-
 x * 22161, calculation from nucleotide sequence Paramecium tetraurelia
3.4.22.15 22500
-
 x * 22500, ion-spray mass spectrometry Paramecium tetraurelia
3.4.22.15 27000
-
 gel filtration Paramecium tetraurelia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.15 additional information Paramecium tetraurelia involved in extracellular food digestion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Paramecium tetraurelia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Paramecium tetraurelia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 azocasein + H2O
-
 Paramecium tetraurelia ?
-
 ?
3.4.22.15 benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O
-
 Paramecium tetraurelia ?
-
 ?
3.4.22.15 glycogen phosphorylase + H2O
-
 Paramecium tetraurelia ?
-
 ?
3.4.22.15 additional information involved in extracellular food digestion Paramecium tetraurelia ?
-
 ?
3.4.22.15 phosphorylase a + H2O
-
 Paramecium tetraurelia ?
-
 ?
3.4.22.15 serum albumin + H2O
-
 Paramecium tetraurelia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 22161, calculation from nucleotide sequence Paramecium tetraurelia
3.4.22.15 ? x * 22500, ion-spray mass spectrometry Paramecium tetraurelia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.15 56
-
-
 Paramecium tetraurelia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 6.5
-
 hydrolysis of phosphorylase a Paramecium tetraurelia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 4.5 9 50% of maximal activity at pH 4.5 and at pH 9.0, hydrolysis of phosphorylase a Paramecium tetraurelia