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Literature summary extracted from

  • Kirschke, H.; Langner, J.; Wiederanders, B.; Ansorge, S.; Bohley, P.
    Cathepsin L. A new proteinase from rat-liver lysosomes (1977), Eur. J. Biochem., 74, 293-301.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 ATP activates Rattus norvegicus
3.4.22.15 CoA activates Rattus norvegicus
3.4.22.15 EDTA activates Rattus norvegicus
3.4.22.15 Soybean trypsin inhibitor activates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
3.4.22.15 Azodicarboxylic acid alpha-morpholide
-
 Rattus norvegicus
3.4.22.15 diazinedicarboxylic acid bisdimethylamide
-
 Rattus norvegicus
3.4.22.15 Hg2+
-
 Rattus norvegicus
3.4.22.15 iodoacetamide
-
 Rattus norvegicus
3.4.22.15 iodoacetic acid
-
 Rattus norvegicus
3.4.22.15 Leu-CH2Cl
-
 Rattus norvegicus
3.4.22.15 leupeptin pseudo-irreversible inhibitor Rattus norvegicus
3.4.22.15 methylphenylazoformate
-
 Rattus norvegicus
3.4.22.15 Mn2+
-
 Rattus norvegicus
3.4.22.15 NEM
-
 Rattus norvegicus
3.4.22.15 PCMB
-
 Rattus norvegicus
3.4.22.15 tosyl-Lys-CH2Cl
-
 Rattus norvegicus
3.4.22.15 tosyl-Phe-CH2Cl
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 lysosome
-
 Rattus norvegicus 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 0.0517
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.22.15 4°C, remarkable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 azocasein + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 benzoyl-Arg-4-nitroanilide + H2O
-
 Rattus norvegicus benzoyl-Arg + 4-nitroaniline
-
 ?
3.4.22.15 Collagen + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 Glucagon + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 Hemoglobin + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 histone + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 insulin + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 N2-benzoyl-L-argininamide + H2O
-
 Rattus norvegicus NH3 + N2-benzoyl-L-arginine
-
 ?
3.4.22.15 Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O
-
 Rattus norvegicus Nalpha-benzoyl-L-Arg + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 23000-24000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 5
-
 hydrolysis of azocasein, glucagon or benzoyl-Arg-NH2 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 3 7 pH 3.0: 40-50% of maximal activity, pH 7.0: 20-30% of maximal activity Rattus norvegicus