Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dufour, E.; Obled, A.; Valin, C.; Bechet, D.; Ribadeau-Dumas, B.; Huet, J.C.
    Purification and amino acid sequence of chicken liver cathepsin L (1987), Biochemistry, 26, 5689-5695.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 thiol-reducing agent required for hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 Benzyloxycarbonyl-Phe-Phe-CHN2
-
 Gallus gallus
3.4.22.15 leupeptin
-
 Gallus gallus
3.4.22.15 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.15 0.006
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 lysosome
-
 Gallus gallus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 23668
-
 x * 23668, calculation from amino acid composition Gallus gallus
3.4.22.15 27000
-
 x * 27000, SDS-PAGE under reducing conditions Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 2500
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 27000, SDS-PAGE under reducing conditions Gallus gallus
3.4.22.15 ? x * 23668, calculation from amino acid composition Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.15 30
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 5 5.5
-
 Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.15 7.4
-
 2 min, 97% loss of activity Gallus gallus