Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grant, S.K.; Deckman, I.C.; Minnich, M.D.; Culp, J.; Franklin, S.; Dreyer, G.B.; Tomaszek, T.A., Jr.; Debouck, C.; Meek, T.D.
    Purification and biochemical characterization of recombinant simian immunodeficiency virus protease and comparison to human immunodeficiency virus type 1 protease (1991), Biochemistry, 30, 8424-8434.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.B2 expressed in Escherichia coli with a recombinant expression system Simian immunodeficiency virus

General Stability

EC Number General Stability Organism
3.4.23.B2 purified enzyme is unstable due to autopropteolysis Simian immunodeficiency virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.B2 1,2-epoxy-3-(4-nitrophenoxy)propane i.e. EPNP; irreversible inactivation, half-maximal inactivation at 8.6 mM Simian immunodeficiency virus
3.4.23.B2 amastatin
-
 Simian immunodeficiency virus
3.4.23.B2 chymostatin
-
 Simian immunodeficiency virus
3.4.23.B2 pepstatin A
-
 Simian immunodeficiency virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.B2 0.3
-
 Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 3.1
-
 acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 4.3
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 4.6
-
 Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 pH 6, 37°C Simian immunodeficiency virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.B2 10812
-
 2 * 10812, electrospray mass spectrometry Simian immunodeficiency virus
3.4.23.B2 11600
-
 2 * 11600, SDS-PAGE Simian immunodeficiency virus
3.4.23.B2 18000 19000 gel filtration Simian immunodeficiency virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.B2 Simian immunodeficiency virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.B2
-
 Simian immunodeficiency virus

Storage Stability

EC Number Storage Stability Organism
3.4.23.B2 -70°C, 50 mM sodium acetate, pH 5.0, 5 mM EDTA, 10 mM DTT, 0.35 NaCl, 40% v/v glycerol Simian immunodeficiency virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.B2 Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Ala-Thr-Leu-Asn-Phe + Pro-Ile-Ser-Pro-Ile-Glu-NH2
-
 ?
3.4.23.B2 Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Arg-Ala-Ser-Gln-Asn-Tyr-OH + Pro-Val-Val-NH2
-
 ?
3.4.23.B2 Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Arg-Lys-Ile-Leu + Phe-Leu-Asp-Gly-NH2
-
 ?
3.4.23.B2 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Ser-Gln-Asn-Tyr + Pro-Val-Val-NH2
-
 ?
3.4.23.B2 acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val + H2O
-
 Simian immunodeficiency virus acetyl-Arg-Ala-Ser-Gln-Asn-Tyr + Pro-Val-Val
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.B2 dimer 2 * 10812, electrospray mass spectrometry Simian immunodeficiency virus
3.4.23.B2 dimer 2 * 11600, SDS-PAGE Simian immunodeficiency virus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.B2 0.6
-
 Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 1.3
-
 Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 6.2
-
 acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 6.4
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 pH 6, 37°C Simian immunodeficiency virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.B2 5.5
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 or Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly as substrate Simian immunodeficiency virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.23.B2 0.003
-
 pepstatin A pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 0.3
-
 chymostatin pH 6, 37°C Simian immunodeficiency virus
3.4.23.B2 0.3
-
 amastatin pH 6, 37°C Simian immunodeficiency virus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.23.B2 Simian immunodeficiency virus isoelectric focusing
-
 8.2