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Literature summary extracted from

  • Bourgeau, G.; Lapointe, H.; Peloquin, P.; Mayrand, D.
    Cloning, expression, and sequencing of a protease gene (tpr) from Porphyromonas gingivalis W83 in Escherichia coli (1992), Infect. Immun., 60, 3186-3192.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B8 2-mercaptoethanol stimulates Porphyromonas gingivalis
3.4.22.B8 cysteine stimulates Porphyromonas gingivalis
3.4.22.B8 dithiothreitol stimulates Porphyromonas gingivalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B8 expression in Escherichia coli Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B8 Ca2+ inhibitory concentration: 15-20 mM Porphyromonas gingivalis
3.4.22.B8 Co2+ inhibitory below 5 mM Porphyromonas gingivalis
3.4.22.B8 Cu2+ inhibitory below 5 mM Porphyromonas gingivalis
3.4.22.B8 EDTA
-
 Porphyromonas gingivalis
3.4.22.B8 Fe2+ inhibitory below 5 mM Porphyromonas gingivalis
3.4.22.B8 Hg2+ inhibitory below 5 mM Porphyromonas gingivalis
3.4.22.B8 Mg2+ inhibitory concentration: 15-20 mM Porphyromonas gingivalis
3.4.22.B8 Mn2+ inhibitory concentration: 5-10 mM Porphyromonas gingivalis
3.4.22.B8 NEM
-
 Porphyromonas gingivalis
3.4.22.B8 Ni2+ inhibitory below 5 mM Porphyromonas gingivalis
3.4.22.B8 PCMB
-
 Porphyromonas gingivalis
3.4.22.B8 TLCK
-
 Porphyromonas gingivalis
3.4.22.B8 TPCK
-
 Porphyromonas gingivalis
3.4.22.B8 Zn2+ inhibitory below 5 mM Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B8 64000
-
 x * 64000, SDS-PAGE Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.B8 additional information Porphyromonas gingivalis the protease constitutes an important virulence factor ?
-
 ?
3.4.22.B8 additional information Porphyromonas gingivalis W83 the protease constitutes an important virulence factor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B8 Porphyromonas gingivalis P25806 W83
-
3.4.22.B8 Porphyromonas gingivalis W83 P25806 W83
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B8 Azocoll + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 Azocoll + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
3.4.22.B8 Bovine serum albumin + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 Bovine serum albumin + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
3.4.22.B8 casein + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 casein + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
3.4.22.B8 denatured collagen + H2O no activity on native collagen Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 additional information no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 additional information the protease constitutes an important virulence factor Porphyromonas gingivalis ?
-
 ?
3.4.22.B8 additional information no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide Porphyromonas gingivalis W83 ?
-
 ?
3.4.22.B8 additional information the protease constitutes an important virulence factor Porphyromonas gingivalis W83 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B8 ? x * 64000, SDS-PAGE Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.B8 45 70
-
 Porphyromonas gingivalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.B8 additional information
-
 inactive at or below 20 C when azocoll is the substrate, inactive at 90 C Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B8 6.5 12
-
 Porphyromonas gingivalis