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Literature summary extracted from

  • Lövgren, J.; Airas, K.; Lilja, H.
    Enzymatic action of human glandular kallikrein 2 (hK2). Substrate specificity and regulation by Zn2+ and extracellular protease inhibitors (1999), Eur. J. Biochem., 262, 781-789.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.35 antipain weak Homo sapiens
3.4.21.35 leupeptin weak Homo sapiens
3.4.21.35 Protein C inhibitor
-
 Homo sapiens
3.4.21.35 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.35 0.02
-
 Ala-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.04
-
 Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.048
-
 Val-Pro-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.071
-
 Leu-Leu-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 additional information Homo sapiens likely the physiological activator of the zymogen form of prostate-specific antigen in the prostate ?
-
 ?
3.4.21.35 polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 prostate gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 Ala-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.35 Leu-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Leu-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 additional information
-
 Homo sapiens ?
-
 ?
3.4.21.35 additional information likely the physiological activator of the zymogen form of prostate-specific antigen in the prostate Homo sapiens ?
-
 ?
3.4.21.35 polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
3.4.21.35 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 pro-urokinase + H2O
-
 Homo sapiens urokinase
-
 ?
3.4.21.35 semenogelin I + H2O
-
 Homo sapiens semenogelin I fragments
-
 ?
3.4.21.35 semenogelin II + H2O
-
 Homo sapiens semenogelin II fragments
-
 ?
3.4.21.35 Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Val-Pro-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 zymogen of prostate-specific antigen + H2O
-
 Homo sapiens prostate-specific antigen + APLILSR
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.35 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.35 0.0267
-
 Val-Pro-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.04
-
 Leu-Leu-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.12
-
 Ala-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
3.4.21.35 0.917
-
 Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 7.5
-
 assay at Homo sapiens
3.4.21.35 8
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.35 6.5 9.5 pH 6.5: about 30% of maximal activity, pH 9.5: about 40% of maximal activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.35 0.000000025
-
 Protein C inhibitor pH 7.5, 37°C Homo sapiens