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Literature summary extracted from
- Rat urinary kallikrein (1988), Methods Enzymol., 163, 128-143.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.35 | bovine serum albumin | 1 mg/ml, activates | Rattus norvegicus | |
| 3.4.21.35 | Brij-58 | 0.1%, activates | Rattus norvegicus |  |
| 3.4.21.35 | deoxycholate | 0.1%, activates | Rattus norvegicus | |
| 3.4.21.35 | Lima-bean trypsin inhibitor | 0.017 mg/ml, activates | Rattus norvegicus | |
| 3.4.21.35 | Lubrol Px | 0.1%, activates | Rattus norvegicus | |
| 3.4.21.35 | ovomucoid trypsin inhibitor | 0.017 mg/ml, activates | Rattus norvegicus | |
| 3.4.21.35 | Triton X-100 | 0.1%, activates | Rattus norvegicus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.35 | freezing and thawing results in at least three-fold increase of specific activity | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.35 | amiloride | - |
Rattus norvegicus | |
| 3.4.21.35 | Aprotinin | - |
Rattus norvegicus | |
| 3.4.21.35 | benzamidine | - |
Rattus norvegicus | |
| 3.4.21.35 | diisopropyl fluorophosphate | at a slow rate | Rattus norvegicus | |
| 3.4.21.35 | kallikrein antibody | - |
Rattus norvegicus | |
| 3.4.21.35 | leupeptin | - |
Rattus norvegicus | |
| 3.4.21.35 | Pentamidine | - |
Rattus norvegicus | |
| 3.4.21.35 | Phe-Phe-Arg-CH2Cl | - |
Rattus norvegicus | |
| 3.4.21.35 | Soybean trypsin inhibitor | - |
Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.35 | extracellular | secretion | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.35 | 38000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.35 | polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.35 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.35 | proteolytic modification | urinary and renal kallikrein exist in both active and proenzyme form | Rattus norvegicus |
| 3.4.21.35 | proteolytic modification | activation by trypsin | Rattus norvegicus |
| 3.4.21.35 | side-chain modification | multiple forms are due at least in part to varying carbohydrates | Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.35 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.35 | kidney | - |
Rattus norvegicus | - |
| 3.4.21.35 | urine | - |
Rattus norvegicus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.35 | -20°C, 0.1 N NaCl, 0.01 M sodium phosphate buffer, pH 7.0, stable for months | Rattus norvegicus |
| 3.4.21.35 | 4°C, stable for weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.35 | D-Val-Leu-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | D-Val-Leu-Arg + p-nitroaniline | - |
? | |
| 3.4.21.35 | kininogen + H2O | high molecular weight kininogen from human and rat | Rattus norvegicus | kinin + ? | - |
? | |
| 3.4.21.35 | kininogen + H2O | cleavage of rat kininogen at Arg-Arg and Arg-Ser | Rattus norvegicus | bradykinin + kinin heavy chain + kinin light chain | - |
? | |
| 3.4.21.35 | low-molecular-weight kininogen + H2O | bovine or human | Rattus norvegicus | kallidin + kinin | - |
? | |
| 3.4.21.35 | polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
| 3.4.21.35 | Tosyl-Arg methyl ester + H2O | - |
Rattus norvegicus | Tosyl-Arg + methanol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.35 | monomer | 1 * 38000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.35 | 25 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.35 | 55 | - |
18 h, 70% of the activity is retained | Rattus norvegicus |
| 3.4.21.35 | 100 | - |
5 min, 60% loss of activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.35 | 7.4 | - |
assay at | Rattus norvegicus |
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