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Literature summary extracted from

  • Ohnishi, N.; Ikekita, M.; Atomi, Y.; Kizuki, K.; Moriya, H.; Kondo, K.; Yamada, H.; Tsugita, A.
    Canine pancreatic kallikrein: enzyme isolation and characterization (1992), Protein Seq. Data Anal., 5, 1-5.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.35 chymostatin
-
 Canis lupus familiaris
3.4.21.35 Soybean trypsin inhibitor kallikrein B is inhibited, kallikrein A not Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.35 11000
-
 x * 11000 + x * 16000, kallikrein A, SDS-PAGE after reduction with 2-mercaptoethanol Canis lupus familiaris
3.4.21.35 16000
-
 x * 4000 + x * 16000, kallikrein B, SDS-PAGE after reduction with 2-mercaptoethanol Canis lupus familiaris
3.4.21.35 16000
-
 x * 11000 + x * 16000, kallikrein A, SDS-PAGE after reduction with 2-mercaptoethanol Canis lupus familiaris
3.4.21.35 27000
-
 x * 27000, kallikrein B, SDS-PAGE Canis lupus familiaris
3.4.21.35 33000
-
 kallikrein B, gel filtration Canis lupus familiaris
3.4.21.35 36000
-
 kallikrein A, gel filtration Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?
3.4.21.35 polypeptide + H2O Canis lupus familiaris
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Canis lupus familiaris
-
 kallikrein A and B
-
3.4.21.35 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.35
-
 Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 pancreas
-
 Sus scrofa
-
3.4.21.35 pancreas
-
 Canis lupus familiaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
3.4.21.35 polypeptide + H2O
-
 Canis lupus familiaris peptides
-
 ?
3.4.21.35 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Sus scrofa Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.35 ? x * 4000 + x * 16000, kallikrein B, SDS-PAGE after reduction with 2-mercaptoethanol Canis lupus familiaris
3.4.21.35 ? x * 33000, kallikrein A, SDS-PAGE Canis lupus familiaris
3.4.21.35 ? x * 27000, kallikrein B, SDS-PAGE Canis lupus familiaris
3.4.21.35 ? x * 11000 + x * 16000, kallikrein A, SDS-PAGE after reduction with 2-mercaptoethanol Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 8.4
-
-
 Canis lupus familiaris