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Literature summary extracted from

  • Bizzozero, S.A.; Dutler, H.
    Comparative specificity of porcine pancreatic kallikrein and bovine pancreatic trypsin. Importance of interactions N-terminal to the scissible bond (1987), Arch. Biochem. Biophys., 256, 662-676.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.35 0.0311
-
 acetyl-Phe-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 0.035
-
 acetyl-Ala-Phe-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 0.087
-
 acetyl-Pro-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.089
-
 acetyl-Pro-Phe-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 0.11
-
 acetyl-Ala-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.15
-
 acetyl-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.17
-
 acetyl-Ala-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.21
-
 acetyl-Pro-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.243
-
 benzyloxycarbonyl-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 0.27
-
 acetyl-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.327
-
 benzyloxycarbonyl-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 1.14
-
 acetyl-Ala-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 1.25
-
 benzyloxycarbonyl-Arg-Ala-NH2 pH 8.0, 25°C Sus scrofa
3.4.21.35 4.1
-
 acetyl-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 8.6
-
 acetyl-Ala-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 14
-
 acetyl-Arg-Ala-NH2 pH 8.0, 25°C Sus scrofa
3.4.21.35 16
-
 acetyl-Arg p-nitroanilide pH 8.0, 25°C Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.35
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 acetyl-Ala-Arg-Ala + H2O
-
 Sus scrofa acetyl-Ala-Arg + L-alanine
-
 ?
3.4.21.35 acetyl-Ala-Arg-Arg methyl ester + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.35 acetyl-Ala-Phe-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Ala-Phe-Arg + methanol
-
 ?
3.4.21.35 acetyl-Ala-Phe-Arg-Ala + H2O
-
 Sus scrofa acetyl-Ala-Phe-Arg + L-alanine
-
 ?
3.4.21.35 acetyl-Ala-Phe-Arg-Ala-NH2 + H2O
-
 Sus scrofa acetyl-Ala-Phe-Arg + Ala-NH2
-
 ?
3.4.21.35 acetyl-Ala-Phe-Arg-p-nitroanilide + H2O
-
 Sus scrofa acetyl-Ala-Phe-Arg + p-nitroaniline
-
 ?
3.4.21.35 acetyl-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Arg + methanol
-
 ?
3.4.21.35 acetyl-Arg-Ala-NH2 + H2O
-
 Sus scrofa acetyl-Arg + Ala-NH2
-
 ?
3.4.21.35 acetyl-Arg-p-nitroanilide + H2O
-
 Sus scrofa acetyl-Arg + p-nitroaniline
-
 ?
3.4.21.35 acetyl-Phe-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Phe-Arg + methanol
-
 ?
3.4.21.35 acetyl-Phe-Arg-Ala + H2O
-
 Sus scrofa acetyl-Phe-Arg + L-alanine
-
 ?
3.4.21.35 acetyl-Phe-Arg-p-nitroanilide + H2O
-
 Sus scrofa acetyl-Phe-Arg + p-nitroaniline
-
 ?
3.4.21.35 acetyl-Pro-Phe-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Pro-Phe-Arg + methanol
-
 ?
3.4.21.35 acetyl-Pro-Phe-Arg-Ala + H2O
-
 Sus scrofa acetyl-Pro-Phe-Arg + L-alanine
-
 ?
3.4.21.35 acetyl-Pro-Phe-Arg-Ala-NH2 + H2O
-
 Sus scrofa acetyl-Pro-Phe-Arg + Ala-NH2
-
 ?
3.4.21.35 acetyl-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Sus scrofa acetyl-Pro-Phe-Arg + p-nitroaniline
-
 ?
3.4.21.35 benzyloxycarbonyl-Arg methyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Arg + methanol
-
 ?
3.4.21.35 benzyloxycarbonyl-Arg-Ala-NH2 + H2O
-
 Sus scrofa benzyloxycarbonyl-Arg + Ala-NH2
-
 ?
3.4.21.35 benzyloxycarbonyl-Arg-p-nitroanilide + H2O
-
 Sus scrofa benzyloxycarbonyl-Arg + p-nitroaniline
-
 ?
3.4.21.35 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
3.4.21.35 Tosyl-Arg methyl ester + H2O
-
 Sus scrofa Tosyl-Arg + methanol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.35 25
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.35 0.014
-
 benzyloxycarbonyl-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.02
-
 acetyl-Arg-Ala-NH2 pH 8.0, 25°C Sus scrofa
3.4.21.35 0.05
-
 acetyl-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.0542
-
 acetyl-Ala-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.113
-
 benzyloxycarbonyl-Arg-Ala-NH2 pH 8.0, 25°C Sus scrofa
3.4.21.35 0.24
-
 acetyl-Pro-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.78
-
 acetyl-Pro-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 0.81
-
 acetyl-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 0.87
-
 acetyl-Ala-Phe-Arg-Ala pH 8.0, 25°C Sus scrofa
3.4.21.35 1.69
-
 acetyl-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa
3.4.21.35 1.71
-
 acetyl-Ala-Phe-Arg-p-nitroanilide pH 8.0, 25°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 8
-
 assay at Sus scrofa