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Literature summary extracted from

  • Murakami-Murofushi, K.; Takahashi, T.; Minowa, Y.; Lino, S.; Takeuchi, T.; Kitagaki-Ogawa, H.; Murofushi, H.; Takahshi, K.
    Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum (1990), J. Biol. Chem., 32, 19898-19903.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.103 Diazoacetyl-D,L-norleucine methyl ester in presence of Cu2+ Physarum polycephalum
3.4.21.103 additional information not: 1,2-epoxy-3-(4-nitrophenoxy)propane Physarum polycephalum
3.4.21.103 pepstatin not Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.103 intracellular
-
 Physarum polycephalum 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.103 23000
-
 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum
3.4.21.103 31000
-
 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum
3.4.21.103 68000
-
 gel filtration Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.103 Physarum polycephalum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.103 glycoprotein the heavy chain contains a carbohydrate moiety composed of 7 residues mannose, 5 glucosamine, 1 fucose and 1 glucose Physarum polycephalum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.103
-
 Physarum polycephalum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.103 additional information
-
-
 Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.103 Hemoglobin + H2O
-
 Physarum polycephalum Hydrolyzed hemoglobin
-
 ?
3.4.21.103 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Physarum polycephalum Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.103 additional information derivatives of basic amino acids and related peptides (proteinase E) Physarum polycephalum ?
-
 ?
3.4.21.103 additional information not: N-acetyl-N-phenylalanyl-3,5-diiodo-L-tyrosine Physarum polycephalum ?
-
 ?
3.4.21.103 Oxidized insulin B-chain + H2O major cleavage sites: Gly8-Ser9 (most susceptible), Leu11-Val12, Cysteic acid19-Gly20 and Phe24-Phe25 Physarum polycephalum Hydrolyzed insulin B-chain
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.103 dimer 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.103 37
-
 assay at Physarum polycephalum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.103 25
-
 pH 3.0-6.0, 48 h, stable Physarum polycephalum
3.4.21.103 37
-
 15 min, 30% loss of activity at pH 8.0, 45% loss of activity at pH 9.0 Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.103 1.7
-
 hemoglobin Physarum polycephalum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.103 1.3 3 pH 1.3: about 25% of activity maximum, pH 3.0: about 35% of activity maximum Physarum polycephalum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.103 3 6
-
 Physarum polycephalum
3.4.21.103 8
-
 37°C, 15 min, 30% loss of activity Physarum polycephalum
3.4.21.103 9
-
 37°C, 15 min, 45% loss of activity Physarum polycephalum