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Literature summary extracted from

  • Halio, S.B.; Bauer, M.W.; Mukund, S.; Adams, M.W.W.; Kelly, R.M.
    Purification and characterization of two functional forms of intracellular protease PfpI from the hyperthermophilic archaeon Pyrococcus furiosus (1997), Appl. Environ. Microbiol., 63, 289-295.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B20 N-lauryl sarcosine 0.01% v/v, increases proteolytic activity by about 50% Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B20 intracellular
-
 Pyrococcus furiosus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.B20 NaCl 100 mM, increases proteolytic activity by about 50% Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B20 18800
-
 3 * 18800, enzyme form PfpI-C2, SDS-PAGE Pyrococcus furiosus
3.4.22.B20 18800
-
 6 * 18800, enzyme form PfpI-C1, SDS-PAGE Pyrococcus furiosus
3.4.22.B20 59000
-
 PfpI-C2, gel filtration Pyrococcus furiosus
3.4.22.B20 124000
-
 PfpI-C1, gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B20 Pyrococcus furiosus Q51732
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B20 purification of two enzyme forms: PfpI-C1 and PfpI-C2 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.B20 additional information
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B20 Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B20 Ala-Phe-Lys-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B20 Leu-Tyr-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B20 hexamer 6 * 18800, enzyme form PfpI-C1, SDS-PAGE Pyrococcus furiosus
3.4.22.B20 trimer 3 * 18800, enzyme form PfpI-C2, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.B20 84
-
 enzyme form PfpI-C1, reaction with Leu-Tyr-7-amido-4-methylcoumarinamide Pyrococcus furiosus
3.4.22.B20 86
-
 enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus
3.4.22.B20 97
-
 enzyme form PfpI-C1, reaction with Ala-Phe-Lys-7-amido-4-methylcoumarinamide Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.B20 50 110 50°C: about 55% of maximal activity, 110°C: about 45% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B20 90
-
 half life is 45 min, in presence of 0.5 M KHPO4 the half-life is extended to 80 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus
3.4.22.B20 95
-
 5 min, no inactivation Pyrococcus furiosus
3.4.22.B20 98
-
 half life is below 30 min, in presence of 0.8 M KHPO4 the half-life is extended to 110 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B20 6.3
-
 enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.B20 5.2 8.5 pH 5.2: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.B20 Pyrococcus furiosus enzyme form PfpI-C1
-
 6.1