Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Halio, S.B.; Blumentals, II; Short, S.A.; Merrill, B.M.; Kelly, R.M.
    Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus (1996), J. Bacteriol., 178, 2605-2612.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B20 expression in Escherichia coli Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B20 Pyrococcus furiosus Q51732
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B20 Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B20 ? x + 18800, homomultimer, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.B20 95
-
 reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide, recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.B20 70 100 70°C: about 70% of maximal activity, 100°C: about 75% of maximal activity, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide, recombinant enzyme Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B20 95
-
 in presence of 2% SDS and 80 nM dithiothreitol does not dissociate the protein, half-life: 19 min Pyrococcus furiosus