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Literature summary extracted from

  • Albrecht, A.M.; Boldizsar, E.; Hutchinson, D.J.
    Carboxypeptidase displaying differential velocity in hydrolysis of methotrexate, 5-methyltetrahydrofolic acid, and leucovorin (1978), J. Bacteriol., 134, 506-513.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.17.11 bovine albumin stabilizes against heat inactivation Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.11 2-mercaptoethanol
-
 Flavobacterium sp.
3.4.17.11 citrate
-
 Flavobacterium sp.
3.4.17.11 CoCl2
-
 Flavobacterium sp.
3.4.17.11 DTT
-
 Flavobacterium sp.
3.4.17.11 EDTA Ca2+, Co2+, Mg2+ and Zn2+ reverse inhibition Flavobacterium sp.
3.4.17.11 L-Glutamic acid weak Flavobacterium sp.
3.4.17.11 potassium ascorbate 1 mM: harmless, 3 mM: strong inhibition Flavobacterium sp.
3.4.17.11 Pteroic acid
-
 Flavobacterium sp.
3.4.17.11 ZnCl2
-
 Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.11 Ca2+ weak activator in absence of EDTA Flavobacterium sp.
3.4.17.11 Zn2+ weak activator in absence of EDTA Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.11 53000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.11 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.11
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.11 39.43
-
-
 Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
3.4.17.11 -20°C, stable for 1 year Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.11 2,4-dihydroxypteroyl-L-glutamic acid + H2O
-
 Flavobacterium sp. 2,4-dihydroxypteroate + L-Glu
-
 ?
3.4.17.11 4-aminopteroylglutamic acid + H2O i.e. aminopterin Flavobacterium sp. 4-aminopteroate + Glu
-
 ?
3.4.17.11 5-formyltetrahydrofolic acid + H2O i.e. leucovorin Flavobacterium sp. 5-formylpteroate + Glu
-
 ?
3.4.17.11 5-methyltetrahydrofolate + H2O weak Flavobacterium sp. 5-methylpteroate + Glu
-
 ?
3.4.17.11 folic acid + H2O
-
 Flavobacterium sp. pteroic acid + glutamic acid
-
 ?
3.4.17.11 methotrexate + H2O
-
 Flavobacterium sp. 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?
3.4.17.11 additional information releases the carboxy-terminal glutamyl moiety of derivatives of pteroyl-mono-L-glutamic acid Flavobacterium sp. ?
-
 ?
3.4.17.11 additional information substituents on N5 of the pteridine ring decrease the velocity of hydrolysis Flavobacterium sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.11 30
-
 with methotrexate and 5-methyltetrahydrofolate as substrates, the reaction proceeds equally well between 30°C and 42°C Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.11 4
-
 half-life: 4 h Flavobacterium sp.
3.4.17.11 25
-
 half-life: 90 min Flavobacterium sp.
3.4.17.11 37
-
 half-life: 45 min Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.11 6 8 hydrolysis of 5-methyltetrahydrofolate, phosphate buffer Flavobacterium sp.
3.4.17.11 6.5 6.8 hydrolysis of leucoverin, phosphate buffer Flavobacterium sp.
3.4.17.11 7 7.5
-
 Flavobacterium sp.
3.4.17.11 7 8 hydrolysis of 5-methyltetrahydrofolate, Tris buffer Flavobacterium sp.
3.4.17.11 7.2
-
 hydrolysis of methotrexate, folic acid Flavobacterium sp.
3.4.17.11 7.5 8 hydrolysis of leucoverin, Tris buffer Flavobacterium sp.