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Literature summary extracted from

  • McCullough, J.L.; Chabner, B.A.; Bertino, J.R.
    Purification and properties of carboxypeptidase G 1 (1971), J. Biol. Chem., 246, 7207-7213.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.11 4-Aminopteroylaspartic acid hydrolysis of 4-amino-N10-methylpteroylglutamic acid Pseudomonas stutzeri
3.4.17.11 L-aspartic acid weak inhibition of hydrolysis of 4-amino-N10-methylpteroylglutamic acid Pseudomonas stutzeri
3.4.17.11 L-Glutamic acid hydrolysis of 4-amino-N10-methylpteroylglutamic acid, no inhibition by D-glutamic acid Pseudomonas stutzeri
3.4.17.11 N-Benzyloxycarbonyl glutamic acid hydrolysis of 4-amino-N10-methylpteroylglutamic acid Pseudomonas stutzeri
3.4.17.11 N-Benzyloxycarbonylaspartic acid hydrolysis of 4-amino-N10-methylpteroylglutamic acid Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.11 0.0011
-
 pteroylglutamic acid
-
 Pseudomonas stutzeri
3.4.17.11 0.0039
-
 methotrexate
-
 Pseudomonas stutzeri
3.4.17.11 0.0083
-
 aminopterin
-
 Pseudomonas stutzeri
3.4.17.11 0.0129
-
 5-methyltetrahydrofolic acid
-
 Pseudomonas stutzeri
3.4.17.11 0.0181
-
 5-formyltetrahydrofolic acid
-
 Pseudomonas stutzeri
3.4.17.11 0.104
-
 4-aminopteroylaspartate
-
 Pseudomonas stutzeri
3.4.17.11 0.58
-
 4-amino-N10-methylpteroylaspartate
-
 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.11 Zn2+ is the only heavy metal ion capable of restoring full activity when the endogenous metal is removed by incubation with the chelating resin Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.11 46000
-
 2 * 46000, SDS-PAGE Pseudomonas stutzeri
3.4.17.11 92000
-
 gel filtration Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.11 Pseudomonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.11
-
 Pseudomonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.11 725
-
-
 Pseudomonas stutzeri

Storage Stability

EC Number Storage Stability Organism
3.4.17.11 -20°C, 0.01 M Tris-chloride buffer, pH 7.3, 0.01 mM ZnCl2, stable for several months Pseudomonas stutzeri
3.4.17.11 4°C, 0.01 M Tris-chloride buffer, pH 7.3, 0.01 mM ZnCl2, stable for 2 to 3 weeks Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.11 4-amino-N10-methylpteroylaspartic acid + H2O
-
 Pseudomonas stutzeri 4-amino-N10-methylpteroate + Asp
-
 ?
3.4.17.11 4-aminobenzoyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri 4-aminobenzoate + glutamate
-
 ?
3.4.17.11 4-aminopteroylaspartic acid + H2O
-
 Pseudomonas stutzeri 4-aminopteroate + Asp
-
 ?
3.4.17.11 4-aminopteroylglutamic acid + H2O i.e. aminopterin Pseudomonas stutzeri 4-aminopteroate + Glu
-
 ?
3.4.17.11 5-formyltetrahydrofolic acid + H2O i.e. leucovorin Pseudomonas stutzeri 5-formylpteroate + Glu
-
 ?
3.4.17.11 5-methyltetrahydrofolate + H2O
-
 Pseudomonas stutzeri 5-methylpteroate + Glu
-
 ?
3.4.17.11 alpha-L-glutamyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri L-Glu + L-Glu
-
 ?
3.4.17.11 aminopterin + H2O
-
 Pseudomonas stutzeri ?
-
 ?
3.4.17.11 benzoyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri benzoate + glutamate
-
 ?
3.4.17.11 dihydrofolic acid + H2O
-
 Pseudomonas stutzeri dihydropteroic acid + Glu
-
 ?
3.4.17.11 folic acid + H2O i.e. pteroyl-L-glutamic acid Pseudomonas stutzeri pteroic acid + glutamic acid
-
 r
3.4.17.11 folic acid + H2O equilibrium lies far towards hydrolysis Pseudomonas stutzeri pteroic acid + glutamic acid
-
 r
3.4.17.11 folic acid + H2O no activity with pteroyl-D-glutamic acid Pseudomonas stutzeri pteroic acid + glutamic acid
-
 r
3.4.17.11 gamma-L-glutamyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri L-Glu + L-Glu
-
 ?
3.4.17.11 glycyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri glycine + glutamate
-
 ?
3.4.17.11 L-tyrosyl-L-glutamic acid + H2O
-
 Pseudomonas stutzeri tyrosine + glutamate
-
 ?
3.4.17.11 methotrexate + H2O i.e. amethopterin Pseudomonas stutzeri 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?
3.4.17.11 additional information hydrolyzes the COOH-terminal glutamate of oligopeptides and N-benzyloxycarbonyl glutamates Pseudomonas stutzeri ?
-
 ?
3.4.17.11 additional information lesser activity against aspartate COOH-terminal peptide linkages Pseudomonas stutzeri ?
-
 ?
3.4.17.11 N-benzyloxycarbonyl-L-aspartic acid + H2O
-
 Pseudomonas stutzeri benzyl carbonate + L-Asp
-
 ?
3.4.17.11 N-benzyloxycarbonyl-L-glutamic acid + H2O no activity with D-glutamic acid Pseudomonas stutzeri benzyl carbonate + L-Glu
-
 ?
3.4.17.11 N-benzyloxycarbonyl-L-glutamine + H2O
-
 Pseudomonas stutzeri benzyl carbonate + L-Gln
-
 ?
3.4.17.11 pteroyl-gamma,gamma-triglutamate + H2O
-
 Pseudomonas stutzeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.11 dimer 2 * 46000, SDS-PAGE Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.11 6.3 7.3
-
 Pseudomonas stutzeri