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Literature summary extracted from

  • Latchinian-Sadek, L.; Thomas, D.Y.
    Expression, purification, and characterization of the yeast KEX1 gene product, a polypeptide precursor processing carboxypeptidase (1993), J. Biol. Chem., 268, 534-540.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.6 1-chloro-3-tosylamido-7-amino-2-heptanone
-
 Saccharomyces cerevisiae
3.4.16.6 alpha-Factor KR
-
 Saccharomyces cerevisiae
3.4.16.6 Enkephalin heptapeptides
-
 Saccharomyces cerevisiae
3.4.16.6 Guanidinoethylmercaptosuccinic acid
-
 Saccharomyces cerevisiae
3.4.16.6 Hg2+
-
 Saccharomyces cerevisiae
3.4.16.6 leupeptin
-
 Saccharomyces cerevisiae
3.4.16.6 phenylmethylsulfonyl fluoride
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.6 0.284
-
 Benzoyl-Phe-Ala-Arg
-
 Saccharomyces cerevisiae
3.4.16.6 0.516
-
 furylacryloyl-Ala-Arg
-
 Saccharomyces cerevisiae
3.4.16.6 0.962
-
 furylacryloyl-Ala-Lys
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.6 Ca2+ activates at low concentrations Saccharomyces cerevisiae
3.4.16.6 Mg2+ activates at low concentrations Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.16.6 additional information Saccharomyces cerevisiae plays a role in polypeptide processing in yeast ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.6 Saccharomyces cerevisiae
-
 KEX1 gene from Saccharomyces cerevisiae expressed using the baculovirus/insect cell system
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.6 KEX1 gene from Saccharomyces cerevisiae expressed using the baculovirus/insect cell system Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.6 22.5
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.6 alpha-factor-Lys-Arg + H2O maturation takes place in sequential manner Saccharomyces cerevisiae mature active alpha-factor + Lys + Arg
-
 ?
3.4.16.6 benzoyl-Phe-Ala-Arg + H2O
-
 Saccharomyces cerevisiae benzoyl-Phe-Ala + Arg
-
 ?
3.4.16.6 furylacryloyl-Ala-Arg + H2O
-
 Saccharomyces cerevisiae furylacryloyl-Ala + Arg
-
 ?
3.4.16.6 furylacryloyl-Ala-Lys + H2O
-
 Saccharomyces cerevisiae furylacryloyl-Ala + Lys
-
 ?
3.4.16.6 additional information plays a role in polypeptide processing in yeast Saccharomyces cerevisiae ?
-
 ?
3.4.16.6 [Leu5]enkephalin-Arg6 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.16.6 [Leu5]enkephalin-Lys6 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.16.6 [Met5]enkephalin-Arg6 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.16.6 [Met5]enkephalin-Lys6 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.6 6
-
-
 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.16.6 Saccharomyces cerevisiae pI-value below pH 3.0
-
 additional information