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Literature summary extracted from

  • Mima, J.; Jung, G.; Onizuka, T.; Ueno, H.; Hayashi, R.
    Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y (2002), Eur. J. Biochem., 269, 3220-3225.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.5 C341F reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
3.4.16.5 C341G reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu and benzyloxycarbonyl-Gly-Phe, somewhat increased ratio with benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
3.4.16.5 C341S reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
3.4.16.5 C341V reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.5 0.07
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 0.16
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 0.19
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 0.26
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 0.32
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 0.41
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 0.45
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 0.57
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 0.83
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 0.89
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 0.98
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 1.1
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, wild-type enzyme anf mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 3
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
3.4.16.5 3.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 3.7
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 4.2
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 5.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 6.7
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 8.4
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 9.1
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
3.4.16.5 19
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.5 wild-type and mutant enzymes Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.5 benzoyl-Gly-beta-phenyllactate + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.16.5 Benzyloxycarbonyl-Ala-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Ala + Phe
-
 ?
3.4.16.5 Benzyloxycarbonyl-Gly-Leu + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Leu
-
 ?
3.4.16.5 Benzyloxycarbonyl-Gly-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Phe
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Leu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.16.5 0.16
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 0.467
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 0.96
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 1.1
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 1.2
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 1.4
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
3.4.16.5 1.9
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 2.1
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
3.4.16.5 2.2
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 2.3
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 2.4
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 3.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 4.6
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 5.5
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
3.4.16.5 7
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 8
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
3.4.16.5 15
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 18
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 92
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
3.4.16.5 93
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.4.16.5 95
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.4.16.5 150
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae