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Literature summary extracted from

  • Du, P.G.; Kato, S.; Li, Y.H.; Maeda, T.; Yamane, T.; Yamamoto, S.; Fujiwara, M.; Yamamoto, Y.; Nishi, K.; Ohkubo, I.
    Rat tripeptidyl peptidase I: molecular cloning, functional expression, tissue localization and enzymatic characterization (2001), Biol. Chem., 382, 1715-1725.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.9
-
Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.14.9 resistant against freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.9 Ala-Ala-Phe-CH2Cl IC50: 0.06 mM Rattus norvegicus
3.4.14.9 diisopropylfluorophosphate IC50: 1.15 mM Rattus norvegicus
3.4.14.9 Diprotin A 0.1 mM, 50% inhibition Rattus norvegicus
3.4.14.9 Hg2+ 1 mM, complete inhibition, IC50: 0.67 mM Rattus norvegicus
3.4.14.9 p-chloromercuribenzene sulfonic acid IC50: 0.27 mM Rattus norvegicus
3.4.14.9 pepstatin 0.1 mM, 15% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.9 0.68
-
Ala-Ala-Phe-7-amido-4-methylcoumarin pH 4.0 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.9 lysosome
-
Rattus norvegicus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.9 46000
-
x * 46000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus
3.4.14.9 280000
-
non-denaturing PAGE Rattus norvegicus
3.4.14.9 290000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.14.9 additional information Rattus norvegicus an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.9
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.9 adrenal gland
-
Rattus norvegicus
-
3.4.14.9 bronchus
-
Rattus norvegicus
-
3.4.14.9 cerebellum activity in neuroglia cells, no activity in small granule cells, Purkinje cells, remified dendrites and myelinated fibers Rattus norvegicus
-
3.4.14.9 cerebrum
-
Rattus norvegicus
-
3.4.14.9 epididymis
-
Rattus norvegicus
-
3.4.14.9 esophagus
-
Rattus norvegicus
-
3.4.14.9 heart
-
Rattus norvegicus
-
3.4.14.9 kidney activity in proximal tubules, distal tubules, collecting tubules and transitional cells, no activity in glomerulus and Bowman‘s capsule Rattus norvegicus
-
3.4.14.9 large intestine
-
Rattus norvegicus
-
3.4.14.9 liver
-
Rattus norvegicus
-
3.4.14.9 lung
-
Rattus norvegicus
-
3.4.14.9 ovary activity in corpus luteum and corpus albicus, no activity in follicles Rattus norvegicus
-
3.4.14.9 pancreas activity in ducts, no activity in acinar cells and islets of Langerhans Rattus norvegicus
-
3.4.14.9 prostate
-
Rattus norvegicus
-
3.4.14.9 salivary gland activity in excretory duct and stereocilia, no activity in glandular acinus and intercalated portion Rattus norvegicus
-
3.4.14.9 seminal vesicle
-
Rattus norvegicus
-
3.4.14.9 small intestine
-
Rattus norvegicus
-
3.4.14.9 spleen activity in red pulp, no activity in white pulp Rattus norvegicus
-
3.4.14.9 stomach
-
Rattus norvegicus
-
3.4.14.9 testis activity in spermatogonia, spermatozoa, Sertoli cells and Leygig cells, no activity in basement cells Rattus norvegicus
-
3.4.14.9 thymus
-
Rattus norvegicus
-
3.4.14.9 trachea
-
Rattus norvegicus
-
3.4.14.9 uterus
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.9 1.583
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.14.9 4°C, pH 4.0, 50 mM sodium acetate buffer, stable for 10-14 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.9 (pGlu)LYENKPRRRPYIL + H2O i.e. neurotensin, 15.0% degradation after 16 h Rattus norvegicus (pGlu)L + YENKPRRRPYIL
-
?
3.4.14.9 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 33.8% degradation within 60 min Rattus norvegicus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
?
3.4.14.9 DRVYIHPF + H2O i.e. angiotensin II, 18.2% degradation after 16 h Rattus norvegicus DRV + YIHPF
-
?
3.4.14.9 DRVYIHPFHL + H2O i.e. angiotensin I, 11.3% degradation after 16 h Rattus norvegicus DRV + YIHPFHL
-
?
3.4.14.9 GKPIPFFRLK + H2O endo-type substrate, 24.5% degradation after 16 h Rattus norvegicus GKPIP + FFRLK
-
?
3.4.14.9 GNLWATGHFM-NH2 + H2O i.e. neuromedin B, complete degradation after 16 h Rattus norvegicus GNL + WATGHFM-NH2
-
?
3.4.14.9 additional information substrates not cleaved after 24 h of digestion: dynorphin, beta-casomorphin-7, Leu-enkephalin, Met-enkephalin, neurokinin-A, LVV-hemorphin, bradykinin Rattus norvegicus ?
-
?
3.4.14.9 additional information an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 Rattus norvegicus ?
-
?
3.4.14.9 RVYIHPF + H2O i.e. angiotensin III, 95.0% degradation after 15 min Rattus norvegicus RVY + IHPF
-
?
3.4.14.9 YGGFLRKYP + H2O i.e. beta-neo-endorphin, 28% degradation after 16 h Rattus norvegicus YGG + FLRKYP
-
?

Subunits

EC Number Subunits Comment Organism
3.4.14.9 ? x * 46000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.9 37
-
hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.9 33.1
-
Ala-Ala-Phe-7-amido-4-methylcoumarin pH 4.0 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.9 3.5 4.5
-
Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.9 3.5 4.5 stable Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.14.9 0.06
-
IC50: 0.06 mM Rattus norvegicus Ala-Ala-Phe-CH2Cl
3.4.14.9 0.27
-
IC50: 0.27 mM Rattus norvegicus p-chloromercuribenzene sulfonic acid
3.4.14.9 0.67
-
1 mM, complete inhibition, IC50: 0.67 mM Rattus norvegicus Hg2+
3.4.14.9 1.15
-
IC50: 1.15 mM Rattus norvegicus diisopropylfluorophosphate