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Literature summary extracted from

  • Rigolet, P.; Mechin, I.; Delage, M.M.; Chich, J.F.
    The structural basis for catalysis and specificity of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis (2002), Structure, 10, 1383-1394.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 expression in Escherichia coli Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.14.5 purified recombinant enzyme, hanging drop method using 5-10% PEG 4000, pH 5.2, 18°C, preparation of heavy atom derivatives, X-ray diffraction structure determination and analysis at 2.2 A resolution Lactococcus lactis
3.4.14.11 hanging drop method Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.11 membrane the C-terminal moiety probably plays a role in tropism of the enzyme towards the cellular membrane Lactococcus lactis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Lactococcus lactis P22346 ssp. cremoris
-
3.4.14.5 Lactococcus lactis Q9CE11 ssp. cremoris
-
3.4.14.11 Lactococcus lactis P22346
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.14.5 release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline active site structure and accessibility Lactococcus lactis
a

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer homodimeric enzyme, crystal structure, overall fold and structure of the dimer and dimer interface, overview Lactococcus lactis
3.4.14.11 More each monomer is composed of four domains. The larger one presents an alpha/beta hydrolase fold and comprises the active site serine. The specificity pocket is mainly built by residues from a small helical domain which is, together with the N-terminal domain, essential for dimerization Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 More enzyme belongs to the SC clan that includes 38 families of peptidases, and is the prototype of the S15 family Lactococcus lactis
3.4.14.5 PepX
-
 Lactococcus lactis
3.4.14.5 X-PDAP
-
 Lactococcus lactis
3.4.14.5 X-prolyl dipeptidyl aminopeptidase
-
 Lactococcus lactis