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Literature summary extracted from

  • Campbell, B.J.
    Renal dipeptidase (1970), Methods Enzymol., 19, 722-729.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.13.19 extended dialysis against nonchelating buffers does not result in loss of activity Sus scrofa
3.4.13.19 no loss of activity upon lyophilization Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.19 1,10-phenanthroline
-
 Sus scrofa
3.4.13.19 acetate
-
 Sus scrofa
3.4.13.19 Br-
-
 Sus scrofa
3.4.13.19 Cd2+
-
 Sus scrofa
3.4.13.19 Cl-
-
 Sus scrofa
3.4.13.19 CN-
-
 Sus scrofa
3.4.13.19 F-
-
 Sus scrofa
3.4.13.19 HCO3-
-
 Sus scrofa
3.4.13.19 I-
-
 Sus scrofa
3.4.13.19 additional information inhibition by monovalent cations in decreasing order CN-, SCN-, N3-, I-, NO3-, HCO3-, Br-, OAc-, Cl-, F- Sus scrofa
3.4.13.19 N3-
-
 Sus scrofa
3.4.13.19 Ni2+
-
 Sus scrofa
3.4.13.19 NO3-
-
 Sus scrofa
3.4.13.19 nucleotides
-
 Sus scrofa
3.4.13.19 phosphate
-
 Sus scrofa
3.4.13.19 SCN-
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.19 1
-
 glycyldehydrophenylalanine
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.19 Co2+ activates Sus scrofa
3.4.13.19 Mn2+ activates Sus scrofa
3.4.13.19 Zinc contains 1 mol of zinc per mol of enzyme Sus scrofa
3.4.13.19 Zinc no absolute specificity for Zn2+ activation Sus scrofa
3.4.13.19 Zinc contains 1 mol of zinc per mol of enzyme of MW 47200 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.19 47200
-
 approach to equilibrium measurement Sus scrofa
3.4.13.19 87000 93000 sedimentation equilibrium measurement, gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.19 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.19
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.19 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.19 additional information
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.4.13.19 frozen, in dilute solution, 0.13 mg/ml Tris-HCl buffer, pH 8, loses two-third of its activity after 6 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.19 Gly-D-Phe + H2O
-
 Sus scrofa Gly + D-Phe
-
 ?
3.4.13.19 Gly-Gly + H2O
-
 Sus scrofa Gly + Gly
-
 ?
3.4.13.19 glycyldehydrophenylalanine + H2O
-
 Sus scrofa ?
-
 ?
3.4.13.19 L-Ala-Gly + H2O
-
 Sus scrofa L-Ala + Gly
-
 ?
3.4.13.19 additional information broad specificity Sus scrofa ?
-
 ?
3.4.13.19 additional information no esterase activity Sus scrofa ?
-
 ?
3.4.13.19 additional information no hydrolysis of proteins Sus scrofa ?
-
 ?
3.4.13.19 additional information no hydrolysis of tripeptides Sus scrofa ?
-
 ?
3.4.13.19 additional information no hydrolysis of leucinamide Sus scrofa ?
-
 ?
3.4.13.19 additional information no reaction with leucinamide Sus scrofa ?
-
 ?
3.4.13.19 additional information requires the N-terminal amino acid of the dipeptide in the L-configuration Sus scrofa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.19 7.6
-
 glycyldehydrophenylalanine Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.13.19 5
-
 inactivation below Sus scrofa
3.4.13.19 8
-
 stable at Sus scrofa