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Literature summary extracted from

  • Ito, M.; Fukui, T.; Kamokari, M.; Saito, T.; Tomita, K.
    Purification and characterization of acetoacetyl-CoA synthetase from rat liver (1984), Biochim. Biophys. Acta, 794, 183-193.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.16 40% loss of activity on freezing and thawing without glycerol Rattus norvegicus
6.2.1.16 in presence of 5% glycerol the enzyme can be frozen and thawed five times without loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.16 AMP 5 mM, 70% inhibition Rattus norvegicus
6.2.1.16 diphosphate 10 mM, 70% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.16 0.008
-
 acetoacetate
-
 Rattus norvegicus
6.2.1.16 0.01
-
 CoA
-
 Rattus norvegicus
6.2.1.16 0.06
-
 ATP
-
 Rattus norvegicus
6.2.1.16 0.075
-
 L-(+)-3-hydroxybutyrate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.16 Cs+ can replace K+ in activation Rattus norvegicus
6.2.1.16 K+ monovalent cation required Rattus norvegicus
6.2.1.16 Mg2+ required Rattus norvegicus
6.2.1.16 Mn2+ can replace Mg2+ in activation Rattus norvegicus
6.2.1.16 additional information absolute requirement for a monovalent cation and a divalent cation Rattus norvegicus
6.2.1.16 NH4+ can replace K+ in activation Rattus norvegicus
6.2.1.16 Ni2+ can replace Mg2+ in activation Rattus norvegicus
6.2.1.16 Rb+ can replace K+ in activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.16 71000
-
 1 * 71000, SDS-PAGE Rattus norvegicus
6.2.1.16 80000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.16 2.3
-
-
 Rattus norvegicus
6.2.1.16 2.63
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.2.1.16 -20°C, 0.1 mg/ml enzyme, 10 mM Tris-HCl, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.16 ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Rattus norvegicus AMP + diphosphate + acetoacetyl-CoA
-
 ?
6.2.1.16 ATP + L-3-hydroxybutanoate + CoA 48% of the activity relative to acetoacetate Rattus norvegicus AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.16 monomer 1 * 71000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.16 6.5 7.4
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.16 6.2 8.6 about 65% of maximal activity at pH 6.2 and 8.6 Rattus norvegicus