Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wetterholm, A.; Haeggström, J.Z.
    Leukotriene A4 hydrolase: an anion activated peptidase (1992), Biochim. Biophys. Acta, 1123, 275-281.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.6 additional information
-
 additional information influence of NaCl and NaSCN on KM Homo sapiens
3.3.2.6 0.1
-
 proline-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.1
-
 lysine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.2
-
 L-arginine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.3
-
 L-leucine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.5
-
 alanine-4-nitroanilide pH 7.6, 22°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.3.2.6 Br- stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens
3.3.2.6 Cl- stimulates peptidase activity, not epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens
3.3.2.6 thiocyanate stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.6 additional information Homo sapiens possible proteolytic function of the enzyme may be limited to the extracellular space ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.6
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.6 leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.6 leukocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.6 L-arginine-4-nitroanilide + H2O
-
 Homo sapiens L-arginine + 4-nitroaniline
-
 ?
3.3.2.6 L-leucine-4-nitroanilide + H2O
-
 Homo sapiens L-leucine + 4-nitroaniline
-
 ?
3.3.2.6 leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 lysine-4-nitroanilide + H2O
-
 Homo sapiens lysine + 4-nitroaniline
-
 ?
3.3.2.6 additional information not: glycine-4-nitroanilide, glutamyl-4-nitroanilide, valine-4-nitroanilide Homo sapiens ?
-
 ?
3.3.2.6 additional information possible proteolytic function of the enzyme may be limited to the extracellular space Homo sapiens ?
-
 ?
3.3.2.6 proline-4-nitroanilide + H2O
-
 Homo sapiens proline + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.6 37
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.3.2.6 22
-
 22°C: 53% of activity maximum, 50°C: 33% of activity maximum Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.6 0.04
-
 lysine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.15
-
 L-leucine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.15
-
 proline-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.15
-
 L-arginine-4-nitroanilide pH 7.6, 22°C Homo sapiens
3.3.2.6 0.15 2.4 leukotriene A4 value depending on NaSCN concentration Homo sapiens
3.3.2.6 0.6
-
 alanine-4-nitroanilide pH 7.6, 22°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.6 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.6 6.5 8.5 about 25% of activity maximum at pH 6.5 and 8.5 Homo sapiens