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Literature summary extracted from

  • Duwat, P.; de Oliveira, R.; Ehrlich, S.D.; Boiteux, S.
    Repair of oxidative DNA damage in gram-positive bacteria: the Lactococcus lactis Fpg protein (1995), Microbiology, 141, 411-417.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.23 fpg-L gene is cloned, sequenced and overexpressed in Escherichia coli BH410, expression in Escherichia coli fpg and mutY mutants suppresses their spontaneous GC-TA mutator phenotype Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 Zn2+ contains one zinc per enzyme molecule Lactococcus lactis
3.2.2.23 Zn2+ contains a single zinc finger motif near the C-terminus Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 30000
-
 active form Lactococcus lactis
3.2.2.23 31300
-
 1 * 31300, calculated from the amino acid sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Lactococcus lactis important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Lactococcus lactis P42371 subspecies cremoris ML3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23 recombinant enzyme, overexpressed in Escherichia coli BH410 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.23 additional information
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Lactococcus lactis ?
-
 ?
3.2.2.23 DNA + H2O important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species Lactococcus lactis ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Lactococcus lactis 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites Lactococcus lactis 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O
-
 Lactococcus lactis 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 monomer 1 * 30000, SDS-PAGE Lactococcus lactis
3.2.2.23 monomer 1 * 31300, calculated from the amino acid sequence Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.23 Fpg-L
-
 Lactococcus lactis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.2.23 Lactococcus lactis
-
-
 9