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Literature summary extracted from

  • Tchou, J.; Kasai, H.; Chung, M.H.; Laval, J.; Grollman, A.P.; Nishimura, S.
    8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity (1991), Proc. Natl. Acad. Sci. USA, 88, 4690-4694.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.23 additional information not inhibited by 1.2 mM 8-oxo-7,8-dihydro-2’-deoxyguanosine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 0.000008
-
 DNA containing 8-hydroxyguanine residues pH 7.5, 20°C, dublex DNA containing a single 8-oxoguanine residue positioned opposite dC Escherichia coli
3.2.2.23 0.000041
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 20°C, dublex DNA containing a single Me-Fapy residue positioned opposite dC Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 KCl stimulates Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O Escherichia coli dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair 8-hydroxyguanine + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O substrate specificity Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O dublex oligodeoxynucleotides containing 8-oxo-7,8-dihydro-2’-deoxyguanosine positioned opposite dC, dG or dT are cleaved, but not opposite dA or single-stranded DNA, cleaves 3’ and 5’ to the modified base Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine-DNA Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O dublex oligodeoxynucleotides containing Me-Fapy Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?
3.2.2.23 additional information not: DNA containing 8-oxo-7,8-dihydro-2’-deoxyadenosine, single-stranded DNA, dublex DNA containing synthetic abasic sites, mismatches containing dG, unmodified DNA Escherichia coli ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 15
-
 assay at, 13mer substrates Escherichia coli
3.2.2.23 20
-
 assay at, 23mer substrates Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.5
-
 assay at Escherichia coli