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Literature summary extracted from
- Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) (2002), Eur. J. Biochem., 269, 4746-4752.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.22 | 36000 | - |
SDS-PAGE | Saccharina japonica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.22 | rRNA + H2O | Saccharina japonica | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.22 | Saccharina japonica | - |
kelp, cryptogamic marine algal plant, A | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.22 | - |
Saccharina japonica |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.2.22 | leaf | - |
Saccharina japonica | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.22 | additional information | - |
specific activity 8067000 U mg-1, 1 unit is the amount of protein necessary to inhibit protein synthesis by 50% in 0.05 ml rabbit reticulocyte lysate | Saccharina japonica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.22 | additional information | also depurinates synthetic SRD RNA | Saccharina japonica | ? | - |
? | |
| 3.2.2.22 | rRNA + H2O | - |
Saccharina japonica | ? | - |
? | |
| 3.2.2.22 | rRNA + H2O | cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA | Saccharina japonica | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.22 | lamjapin | - |
Saccharina japonica |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.2.22 | Saccharina japonica | isoelectric focusing | - |
8.45 |
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Release 2023.1 (January 2023)
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