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Literature summary extracted from

  • Birkeland, N.K.; Anensen, H.; Knaevelsrud, I.; Kristoffersen, W.; Bjoras, M.; Robb, F.T.; Klungland, A.; Bjelland, S.
    Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus (2002), Biochemistry, 41, 12697-12705.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.21 3-Methyladenine 5 mM, enzyme activity 110% Archaeoglobus fulgidus
3.2.2.21 3-Methylguanine 5 mM, enzyme activity 120% Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.21 gene afalkA, AF2117 ORF cloned and overexpressed in Escherichia coli, gene function suppresses the alkylation sensitivity in Escherichia coli tag alkA double mutants Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.21 additional information resistant to product inhibition by free 3-methyladenine Archaeoglobus fulgidus
3.2.2.21 additional information resistant to product inhibition by free 3-methyladenine Escherichia coli
3.2.2.21 p-hydroxymercuribenzoate
-
Archaeoglobus fulgidus
3.2.2.21 ZnSO4 2 mM, enzyme activity 23% Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.21 Ca2+ 2 mM CaCl2, enzyme activity 103% Archaeoglobus fulgidus
3.2.2.21 Mg2+
-
Escherichia coli
3.2.2.21 Mg2+ 2 mM MgSO4, enzyme activity 112% Archaeoglobus fulgidus
3.2.2.21 Mn2+ 2 mM MnCl2, enzyme activity 102% Archaeoglobus fulgidus
3.2.2.21 Zn2+ 0.1 mM ZnSO4, enzyme activity 114% Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.21 34250
-
predicted from nucleotide sequence Archaeoglobus fulgidus
3.2.2.21 40000
-
recombinant protein, expressed in Escherichia coli, SDS-PAGE Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.21 alkylated DNA + H2O Escherichia coli
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
3.2.2.21 alkylated DNA + H2O Archaeoglobus fulgidus
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.21 Archaeoglobus fulgidus
-
-
-
3.2.2.21 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.21
-
Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.21 additional information
-
specific activity 1100 pmol of 3-methyladenine released/mg of protein, 6.7 mg protein/4 ml Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.21 alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
3.2.2.21 alkylated DNA + H2O
-
Archaeoglobus fulgidus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
3.2.2.21 alkylated DNA + H2O
-
Archaeoglobus fulgidus 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA
-
?
3.2.2.21 additional information
-
Escherichia coli ?
-
?
3.2.2.21 additional information excises alkylated bases only Archaeoglobus fulgidus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.21 MPG
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.21 65 75
-
Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.21 20 80
-
Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.21 45
-
activity reduced by 50% Archaeoglobus fulgidus
3.2.2.21 90
-
60% enzyme activity retained after incubation for 1 h Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.21 5.2
-
-
Archaeoglobus fulgidus