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Literature summary extracted from

  • Fu, J.Y.; Wu, S.M.; Chang, C.T.; Sung, H.Y.
    Characterization of three chitosanase isozymes isolated from a commercial crude porcine pepsin preparation (2003), J. Agric. Food Chem., 51, 1042-1048.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information KM is 5.2 mg/ml for PSC-I, 4 mg/ml for PSC-II and 5.6 mg/ml for PSC-III Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 31000
-
 enzyme from males and winged females Macrotermes estherae
3.2.1.132 35000
-
 enzyme from soldiers Macrotermes estherae
3.2.1.132 37500
-
 gel filtration, isoform PSC-I Sus scrofa
3.2.1.132 40000
-
 enzyme from workers Macrotermes estherae
3.2.1.132 40000
-
 1 * 40000, SDS-PAGE Sus scrofa
3.2.1.132 40600
-
 gel filtration, isoform PSC-II Sus scrofa
3.2.1.132 43700
-
 gel filtration, isoform PSC-III Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Sus scrofa
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Macrotermes estherae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Macrotermes estherae
-
 workers, soldiers, males, winged females
-
3.2.1.132 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 additional information in crude pepsin preparation Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 489.8
-
 isoenzyme PSC-II Sus scrofa
3.2.1.132 733.5
-
 isoenzyme PSC-III Sus scrofa
3.2.1.132 742.6
-
 isoenzyme PSC-I Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Sus scrofa ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Macrotermes estherae ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Macrotermes estherae additional information
-
 ?
3.2.1.132 chitosan + H2O most effective with 68-88% deacetylated chitosan as substrate Sus scrofa additional information
-
 ?
3.2.1.132 N,N'',N'''-triacetylchitotriose + H2O
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 40000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 23 25 enzyme from soldiers Macrotermes estherae
3.2.1.132 24
-
 enzyme from males Macrotermes estherae
3.2.1.132 24 26 enzyme from workers Macrotermes estherae
3.2.1.132 25
-
 enzyme from winged females Macrotermes estherae
3.2.1.132 30
-
 PSC-III Sus scrofa
3.2.1.132 40
-
 PSC-I and PSC-II Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 20 70 all three isoforms are stable in this range for 30 min Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 2.3
-
 chitosan 40°C, pH 4, isoform PSC-II Sus scrofa
3.2.1.132 5.9
-
 chitosan 40°C, pH 4, isoform PSC-I Sus scrofa
3.2.1.132 6.2
-
 chitosan 40°C, pH 4, isoform PSC-III Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 4
-
 isoenzyme PSC-III Sus scrofa
3.2.1.132 4.9
-
 enzyme from males Macrotermes estherae
3.2.1.132 5
-
 isoenzymes PSC-I and PSC-II Sus scrofa
3.2.1.132 5.3
-
 enzyme from winged females Macrotermes estherae
3.2.1.132 5.8
-
 enzyme from soldiers Macrotermes estherae
3.2.1.132 6.2
-
 enzyme from workers Macrotermes estherae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 6 9 isoenzyme PSC-III is stable in this range Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 3 7 isoenzyme PSC-II is stable in this range Sus scrofa
3.2.1.132 5 8 isoenzyme PSC-I is stable in this range Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Sus scrofa isoelectrofocusing, PSC-III
-
 4.4
3.2.1.132 Sus scrofa isoelectrofocusing, PSC-II 4.7 4.6
3.2.1.132 Sus scrofa isoelectrofocusing, PSC-I
-
 4.9