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Literature summary extracted from

  • Jo, Y.Y.; Jo, K.J.; Jin, Y.L.; Kim, K.Y.; Shim, J.H.; Kim, Y.W.; Park, R.D.
    Characterization and kinetics of 45 kDa chitosanase from Bacillus sp. P16 (2003), Biosci. Biotechnol. Biochem., 67, 1875-1882.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.132 acetone, n-butanol and ethylacetate rapidly denature the enzyme Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 2-Hydroxy-5-nitrobenzylbromide
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Ag+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Chloramine T
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 glucosamine
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Hg2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Pb2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Sn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information 0.52 mg/ml with 20.2% acetylated chitosan as substrate Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 K+ slight activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 42000
-
 gel filtration Bacillus sp. (in: Bacteria)
3.2.1.132 45000
-
 1 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria) K-1
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria) P16
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.132 DMSO relativly stable at the concentration of 30% at 37°C for 30 min Bacillus sp. (in: Bacteria)
3.2.1.132 Ethanol relative stable at the concentration of 30% at 37°C for 30 min Bacillus sp. (in: Bacteria)
3.2.1.132 Methanol relative stable at the concentration of 30% at 37°C for 30 min Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. (in: Bacteria)
-
 K-1
-
3.2.1.132 Bacillus sp. (in: Bacteria)
-
 P16
-
3.2.1.132 Bacillus sp. (in: Bacteria) K-1
-
 K-1
-
3.2.1.132 Bacillus sp. (in: Bacteria) P16
-
 P16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 P16 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
3.2.1.132 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) P16 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) P16 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 additional information
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) P16 additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4.5 10
-
 Bacillus sp. (in: Bacteria)