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Literature summary extracted from

  • Eom, T.K.; Lee, K.M.
    Characteristics of chitosanases from Aspergillus fumigatus KB-1 (2003), Arch. Pharm. Res., 26, 1036-1041.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Bi2+ 48% inhibition of chitosanase I Aspergillus fumigatus
3.2.1.132 Cu2+ 59% inhibition of chitosanase I and 48% inhibition of chitosanase II Aspergillus fumigatus
3.2.1.132 Fe2+ 50% inhibition of chitosanase I Aspergillus fumigatus
3.2.1.132 Fe3+ 49% inhibition of chitosanase I Aspergillus fumigatus
3.2.1.132 Hg2+ 81% inhibition of chitosanase I and 80% inhibition of chitosanase II Aspergillus fumigatus
3.2.1.132 Mn2+ 42% inhibition of chitosanase I Aspergillus fumigatus
3.2.1.132 Mo2+ 94% inhibition of chitosanase I and 88% inhibition of chitosanase II Aspergillus fumigatus
3.2.1.132 Pb2+ 69% inhibition of chitosanase I Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 23380
-
 mass spectrometry, chitosanase II Aspergillus fumigatus
3.2.1.132 111200
-
 mass spectrometry, chitosanase I Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Aspergillus fumigatus
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Aspergillus fumigatus KB-1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Aspergillus fumigatus
-
 KB-1
-
3.2.1.132 Aspergillus fumigatus KB-1
-
 KB-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus KB-1 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus additional information chitosanase I produced glucosamine, chitosanase II produced chitooligosaccharides ?
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus KB-1 additional information chitosanase I produced glucosamine, chitosanase II produced chitooligosaccharides ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
 chitosanase I Aspergillus fumigatus
3.2.1.132 70
-
 chitosanase II Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132
-
 60 chitosanase II Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 10
-
 most stable at 10°C, stability of chitosanase I droppes at higher temperatures and most activity is lost above 60°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5
-
 chitosanase II Aspergillus fumigatus
3.2.1.132 6.5
-
 chitosanase I Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 3.5 8.5 chitosanase II Aspergillus fumigatus