EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.123 | Nonidet P-40 | activates | Rhodococcus sp. | |
3.2.1.123 | sodium cholate | - |
Rhodococcus sp. | |
3.2.1.123 | sodium taurodeoxycholate | activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v | Rhodococcus sp. | |
3.2.1.123 | Triton X-100 | activates | Rhodococcus sp. | |
3.2.1.123 | Triton X-100 | activates endoglyceroceramidase II at 0.2-0.4% | Rhodococcus sp. | |
3.2.1.123 | Triton X-100 | activates endoglyceroceramidase I at 0.3-0.4% | Rhodococcus sp. |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.123 | unstable at protein concentration below 0.2 mg/ml or without Triton X-100 | Rhodococcus sp. |
3.2.1.123 | unstable to repeated freezing and thawing | Rhodococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.123 | Ag+ | 1 mM, 63% inhibition of endoglycoceramidase I, 94% inhibition of endoglycoceramidase II | Rhodococcus sp. | |
3.2.1.123 | Brij 58 | - |
Rhodococcus sp. | |
3.2.1.123 | Cu2+ | - |
Rhodococcus sp. | |
3.2.1.123 | Hg2+ | - |
Rhodococcus sp. | |
3.2.1.123 | Tween 20 | - |
Rhodococcus sp. | |
3.2.1.123 | Zn2+ | 1 mM, 20% inhibition of endoglycoceramidase I, 48% inhibition of endoglycoceramidase II | Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.123 | 0.1 | - |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide | pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase I | Rhodococcus sp. | |
3.2.1.123 | 0.21 | - |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide | pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase II | Rhodococcus sp. | |
3.2.1.123 | 0.23 | - |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide | pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase I | Rhodococcus sp. | |
3.2.1.123 | 0.5 | - |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide | pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase II | Rhodococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | 32700 | - |
endoglycoceramidase I, gel filtration | Rhodococcus sp. |
3.2.1.123 | 43000 | - |
endoglycoceramidase II, gel filtration | Rhodococcus sp. |
3.2.1.123 | 55900 | - |
endoglycoceramidase I, PAGE in absence and presence of SDS | Rhodococcus sp. |
3.2.1.123 | 58000 | - |
1 * 58000, SDS-PAGE, endoglyceroceramidase II | Rhodococcus sp. |
3.2.1.123 | 58900 | - |
endoglycoceramidase II, PAGE in absence and presence of SDS | Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.123 | Rhodococcus sp. | - |
2 molecular species: I | - |
3.2.1.123 | Rhodococcus sp. | - |
molecular species: II | - |
3.2.1.123 | Rhodococcus sp. | - |
mutant strain M750 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.123 | 2 molecular species: EGCaseI and EGCaseII, homogeneity | Rhodococcus sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.123 | culture medium | - |
Rhodococcus sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | 23.5 | - |
isoform I | Rhodococcus sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.123 | 0°C, protein concentration greater than 0.2 mg/ml, 50 mM sodium acetate buffer at pH 6.0 or 20 mM phosphate buffer at pH 7.2, 0.1-1.0% Triton X-100, stable for several months | Rhodococcus sp. |
3.2.1.123 | frozen, stable for at least 6 months | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.123 | Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. Gb3Cer | Rhodococcus sp. | Galalpha(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. asialo-GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. lactosylceramide | Rhodococcus sp. | Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. Gb5Cer | Rhodococcus sp. | GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. Gb4Cer | Rhodococcus sp. | GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | glycosphingolipid + H2O | strongly resistant to hydrolysis by endoglycoceramidase II in comparison with endoglycoceramidase I | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | glycosphingolipid + H2O | ganglio-type | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | glycosphingolipid + H2O | neutral and acidic in presence of 0.1% Triton X-100, lacto-type | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | additional information | no substrate: sulfatides | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | additional information | no substrate: sphingomyelins | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | additional information | no substrate: gala-type glycosphingolipids | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | additional information | no substrate: cerebrosides | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | additional information | no substrate: glycoglycerolipids | Rhodococcus sp. | ? | - |
? | |
3.2.1.123 | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM3 | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide | - |
? | |
3.2.1.123 | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.123 | monomer | 1 * 55900, SDS-PAGE, endoglyceroceramidase I | Rhodococcus sp. |
3.2.1.123 | monomer | 1 * 58000, SDS-PAGE, endoglyceroceramidase II | Rhodococcus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | 37 | - |
60 min, pH 3, 50.4% of endoglycoceramidase I activity and 27.5% of endoglycoceramidase II activity lost | Rhodococcus sp. |
3.2.1.123 | 37 | - |
60 min pH 4-8, endoglycoceramidase I and II, stable | Rhodococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | 5 | - |
endoglycoceramidase I | Rhodococcus sp. |
3.2.1.123 | 5.5 | - |
endoglycoceramidase II | Rhodococcus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | additional information | - |
- |
Rhodococcus sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.123 | 4 | 8 | stable for 60 min | Rhodococcus sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.123 | Rhodococcus sp. | endoglycoceramidase I | - |
3.5 |
3.2.1.123 | Rhodococcus sp. | endoglycoceramidase II | - |
4.5 |