Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ito, M.; Yamagata, T.
    Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities (1989), J. Biol. Chem., 264, 9510-9519.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.123 Nonidet P-40 activates Rhodococcus sp.
3.2.1.123 sodium cholate
-
Rhodococcus sp.
3.2.1.123 sodium taurodeoxycholate activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v Rhodococcus sp.
3.2.1.123 Triton X-100 activates Rhodococcus sp.
3.2.1.123 Triton X-100 activates endoglyceroceramidase II at 0.2-0.4% Rhodococcus sp.
3.2.1.123 Triton X-100 activates endoglyceroceramidase I at 0.3-0.4% Rhodococcus sp.

General Stability

EC Number General Stability Organism
3.2.1.123 unstable at protein concentration below 0.2 mg/ml or without Triton X-100 Rhodococcus sp.
3.2.1.123 unstable to repeated freezing and thawing Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.123 Ag+ 1 mM, 63% inhibition of endoglycoceramidase I, 94% inhibition of endoglycoceramidase II Rhodococcus sp.
3.2.1.123 Brij 58
-
Rhodococcus sp.
3.2.1.123 Cu2+
-
Rhodococcus sp.
3.2.1.123 Hg2+
-
Rhodococcus sp.
3.2.1.123 Tween 20
-
Rhodococcus sp.
3.2.1.123 Zn2+ 1 mM, 20% inhibition of endoglycoceramidase I, 48% inhibition of endoglycoceramidase II Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.123 0.1
-
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase I Rhodococcus sp.
3.2.1.123 0.21
-
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase II Rhodococcus sp.
3.2.1.123 0.23
-
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase I Rhodococcus sp.
3.2.1.123 0.5
-
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase II Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.123 32700
-
endoglycoceramidase I, gel filtration Rhodococcus sp.
3.2.1.123 43000
-
endoglycoceramidase II, gel filtration Rhodococcus sp.
3.2.1.123 55900
-
endoglycoceramidase I, PAGE in absence and presence of SDS Rhodococcus sp.
3.2.1.123 58000
-
1 * 58000, SDS-PAGE, endoglyceroceramidase II Rhodococcus sp.
3.2.1.123 58900
-
endoglycoceramidase II, PAGE in absence and presence of SDS Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus sp.
-
2 molecular species: I
-
3.2.1.123 Rhodococcus sp.
-
molecular species: II
-
3.2.1.123 Rhodococcus sp.
-
mutant strain M750
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123 2 molecular species: EGCaseI and EGCaseII, homogeneity Rhodococcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.123 culture medium
-
Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.123 23.5
-
isoform I Rhodococcus sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.123 0°C, protein concentration greater than 0.2 mg/ml, 50 mM sodium acetate buffer at pH 6.0 or 20 mM phosphate buffer at pH 7.2, 0.1-1.0% Triton X-100, stable for several months Rhodococcus sp.
3.2.1.123 frozen, stable for at least 6 months Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb3Cer Rhodococcus sp. Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Rhodococcus sp. Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb5Cer Rhodococcus sp. GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb4Cer Rhodococcus sp. GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 glycosphingolipid + H2O strongly resistant to hydrolysis by endoglycoceramidase II in comparison with endoglycoceramidase I Rhodococcus sp. ?
-
?
3.2.1.123 glycosphingolipid + H2O ganglio-type Rhodococcus sp. ?
-
?
3.2.1.123 glycosphingolipid + H2O neutral and acidic in presence of 0.1% Triton X-100, lacto-type Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: sulfatides Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: sphingomyelins Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: gala-type glycosphingolipids Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: cerebrosides Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: glycoglycerolipids Rhodococcus sp. ?
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.123 monomer 1 * 55900, SDS-PAGE, endoglyceroceramidase I Rhodococcus sp.
3.2.1.123 monomer 1 * 58000, SDS-PAGE, endoglyceroceramidase II Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.123 37
-
60 min, pH 3, 50.4% of endoglycoceramidase I activity and 27.5% of endoglycoceramidase II activity lost Rhodococcus sp.
3.2.1.123 37
-
60 min pH 4-8, endoglycoceramidase I and II, stable Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 5
-
endoglycoceramidase I Rhodococcus sp.
3.2.1.123 5.5
-
endoglycoceramidase II Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.123 additional information
-
-
Rhodococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.123 4 8 stable for 60 min Rhodococcus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.123 Rhodococcus sp. endoglycoceramidase I
-
3.5
3.2.1.123 Rhodococcus sp. endoglycoceramidase II
-
4.5