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Literature summary extracted from

  • Dunne, M.W.; Buckmire, F.L.A.
    Partial purification and characterization of a polymannuronic acid depolymerase produced by a mycoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis (1985), Appl. Environ. Microbiol., 50, 562-567.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.121 CaCl2 at 17 mM inhibition Pseudomonas aeruginosa
3.2.1.121 EDTA 0.5 mM: 35% inhibition Pseudomonas aeruginosa
3.2.1.121 KCl 50 mM: 50% inhibition Pseudomonas aeruginosa
3.2.1.121 MgCl2 at 9 mM inhibition Pseudomonas aeruginosa
3.2.1.121 MgSO4 at 14 mM inhibition Pseudomonas aeruginosa
3.2.1.121 NaCl 50 mM: 40% inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.121 0.096
-
 polymannuronic acid exopolysaccharide 37°C, pH 7.5, from Pseudomonas aeruginosa, strain PM1 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.121 extracellular
-
 Pseudomonas aeruginosa
-
-
3.2.1.121 membrane associated with outer cell membrane Pseudomonas aeruginosa 16020
-
3.2.1.121 periplasm
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.121 CaCl2 5 mM: slight stimulation, at 17 mM inhibition Pseudomonas aeruginosa
3.2.1.121 KCl 3 mM: stimulation, above 10 mM: inhibition Pseudomonas aeruginosa
3.2.1.121 MgCl2 3 mM: slight stimulation, at 9 mM inhibition Pseudomonas aeruginosa
3.2.1.121 MgSO4 3 mM: slight stimulation, at 14 mM inhibition Pseudomonas aeruginosa
3.2.1.121 NaCl 3 mM: stimulation, above 10 mM: inhibition Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.121 polymannuronic acid exopolysaccharide + H2O Pseudomonas aeruginosa from Pseudomonas aeruginosa, strain PM1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.121 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.121 partial Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.121 0.8
-
 sonicated cells with EDTA Pseudomonas aeruginosa
3.2.1.121 2
-
 sonicated cells without EDTA Pseudomonas aeruginosa
3.2.1.121 3.8
-
 supernatant of cells with EDTA Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.121 alginic acid + H2O little activity Pseudomonas aeruginosa ?
-
 ?
3.2.1.121 polymannuronic acid exopolysaccharide + H2O from Pseudomonas aeruginosa, strain PM1 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.121 More major band: 53000 Da, minor band: 23000 Da, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.121 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.121 8
-
-
 Pseudomonas aeruginosa