EC Number | General Stability | Organism |
---|---|---|
3.2.1.113 | enzyme loses a membrane-spanning domain during purification which does not alter its catalytic properties | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.113 | 1-deoxymannojirimycin | strong | Sus scrofa | |
3.2.1.113 | additional information | - |
Sus scrofa | |
3.2.1.113 | N-5-Carboxypentyldeoxymannojirimycin | strong | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.113 | additional information | - |
additional information | kinetic constants of various substrate derivatives | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.113 | endoplasmic reticulum | - |
Sus scrofa | 5783 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.113 | 49000 | - |
1 * 49000, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | Sus scrofa | - |
(Man)5GlcNAc + 4 D-mannose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.113 | Sus scrofa | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.113 | liver | - |
Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Sus scrofa | (Man)5GlcNAc + 4 D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | removes 3 of the 4 alpha1,2-linked mannosyl residues | Sus scrofa | (Man)6(GlcNAc)2 + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | intact chitobiose core affects Man9-mannosidase specificity: reduction or removal of terminal N-acetylglucosamine residue increases hydrolytic susceptibility of the fourth alpha1,2-mannosyl-linkage | Sus scrofa | (Man)6(GlcNAc)2 + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-hexapeptide + H2O | substrate specificity not influenced by peptide moiety, removes 3 mannose residues | Sus scrofa | (Man)6(GlcNAc)2-hexapeptide + D-mannose | - |
? | |
3.2.1.113 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O | very poor substrate | Sus scrofa | (Man)5(GlcNAc)2 + D-mannose | - |
? | |
3.2.1.113 | additional information | no substrate: 4-methylumbelliferyl-alpha-mannoside | Sus scrofa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.113 | monomer | 1 * 49000, SDS-PAGE | Sus scrofa |