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Literature summary extracted from

  • Schweden, J.; Bause, E.
    Characterization of trimming Man9-mannosidase from pig liver. Purification of a catalytically active fragment and evidence for the transmembrane nature of the intact 65 kDa enzyme (1989), Biochem. J., 264, 347-355.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.113 native enzyme protein is highly susceptible to proteolytic cleavage, not by glucopeptidase F Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin
-
 Sus scrofa
3.2.1.113 Basic sugar analogs of mannose strong Sus scrofa
3.2.1.113 Cu2+
-
 Sus scrofa
3.2.1.113 additional information no inhibition by swainsonine; no or little inhibition by N,N-dimethyl-1-deoxymannojirimycin Sus scrofa
3.2.1.113 N-Methyl-deoxymannojirimycin
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 membrane
-
 Sus scrofa 16020
-
3.2.1.113 microsome trans-membrane protein with cytosolic domain Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Ca2+ activation Sus scrofa
3.2.1.113 Ca2+ most effective cation Sus scrofa
3.2.1.113 Mg2+ activation Sus scrofa
3.2.1.113 Mg2+ less effective than Ca2+ Sus scrofa
3.2.1.113 Zn2+ activation, less effective than Ca2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.113 49000
-
 1 * 49000, SDS-PAGE Sus scrofa
3.2.1.113 65000
-
 detected in freshly prepared crude microsomal extracts using polyclonal antibodies, during purification the native enzyme, MW 65000, loses a membrane-spanning domain without losing its catalytic activity Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 affinity chromatography on immobilized N-5-carboxypentyl-1-deoxymannojirimycin Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O removes 3 of the 4 alpha1,2-linked mannosyl residues Sus scrofa (Man)6(GlcNAc)2 + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O highly active on alpha(1-2) linked mannooligosaccharides Sus scrofa (Man)6(GlcNAc)2 + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Sus scrofa ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Sus scrofa ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O very poor substrate Sus scrofa (Man)5(GlcNAc)2 + D-mannose
-
 ?
3.2.1.113 additional information no substrate: 4-methylumbelliferyl-alpha-mannoside Sus scrofa ?
-
 ?
3.2.1.113 additional information pig liver enzyme substrate specificity resembles that of calf liver enzyme Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 monomer 1 * 49000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 6
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.113 5.2 6.7 about half-maximal activity at pH 5.2 and 6.7 Sus scrofa