BRENDA - Enzyme Database

Purification of an almond emulsin fucosidase on Cibacron blue-Sepharose and demonstration of its activity toward fucose-containing glycoproteins

Imbert, M.J.; Glasgow, L.R.; Pizzo, S.V.; J. Biol. Chem. 257, 8205-8210 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.1.111
decreasing ionic strength inactivates
Prunus dulcis
3.2.1.111
freeze-thawing results in 30% loss of initial activity
Prunus dulcis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.111
Hg2+
strong
Prunus dulcis
3.2.1.111
L-fucose
-
Prunus dulcis
3.2.1.111
malonate
above 20 mM
Prunus dulcis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.111
Ca2+
slight activation
Prunus dulcis
3.2.1.111
Mg2+
slight activation
Prunus dulcis
3.2.1.111
additional information
no activation by Mn2+
Prunus dulcis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.111
73000
-
gel filtration
Prunus dulcis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.111
Prunus dulcis
-
almond
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.111
affinity chromatography on Blue Sepharose; partial
Prunus dulcis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.111
emulsin
commercially available
Prunus dulcis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.111
0.0645
-
-
Prunus dulcis
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.111
4░C, less than 10% loss of activity within 6 months
Prunus dulcis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.111
asialoorosomucoid + H2O
specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches
646567
Prunus dulcis
?
-
-
-
?
3.2.1.111
lactoferrin + H2O
hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine
646567
Prunus dulcis
?
-
-
-
?
3.2.1.111
additional information
no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose
646567
Prunus dulcis
?
-
-
-
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.2.1.111
37
-
assay at
Prunus dulcis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.111
5.3
-
-
Prunus dulcis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.111
4.3
5.8
about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8
Prunus dulcis
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.111
decreasing ionic strength inactivates
Prunus dulcis
3.2.1.111
freeze-thawing results in 30% loss of initial activity
Prunus dulcis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.111
Hg2+
strong
Prunus dulcis
3.2.1.111
L-fucose
-
Prunus dulcis
3.2.1.111
malonate
above 20 mM
Prunus dulcis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.111
Ca2+
slight activation
Prunus dulcis
3.2.1.111
Mg2+
slight activation
Prunus dulcis
3.2.1.111
additional information
no activation by Mn2+
Prunus dulcis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.111
73000
-
gel filtration
Prunus dulcis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.111
affinity chromatography on Blue Sepharose; partial
Prunus dulcis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.111
emulsin
commercially available
Prunus dulcis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.111
0.0645
-
-
Prunus dulcis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.111
4░C, less than 10% loss of activity within 6 months
Prunus dulcis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.111
asialoorosomucoid + H2O
specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches
646567
Prunus dulcis
?
-
-
-
?
3.2.1.111
lactoferrin + H2O
hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine
646567
Prunus dulcis
?
-
-
-
?
3.2.1.111
additional information
no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose
646567
Prunus dulcis
?
-
-
-
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.2.1.111
37
-
assay at
Prunus dulcis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.111
5.3
-
-
Prunus dulcis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.111
4.3
5.8
about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8
Prunus dulcis