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Literature summary extracted from

  • Kingston-Smith, A.H.; Major, I.; Parry, M.A.J.; Keys, A.J.
    Purification and properties of a phosphatase in French bean (Phaseolus vulgaris L.) leaves that hydrolyses 2-carboxy-D-arabinitol 1-phosphate (1992), Biochem. J., 287, 821-825.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.63 D-fructose 1,6-bisphosphate activation Phaseolus vulgaris
3.1.3.63 D-fructose 1,6-bisphosphate 2 mM Phaseolus vulgaris
3.1.3.63 additional information no activation by dithiothreitol Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.63 0.43
-
 2-carboxyarabinitol 1-phosphate 25°C, pH 7.5 Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.63 chloroplast
-
 Phaseolus vulgaris 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.63 (NH4)2SO4 activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris
3.1.3.63 CaCl2 activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris
3.1.3.63 KCl activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris
3.1.3.63 MgCl2 activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris
3.1.3.63 NaCl activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris
3.1.3.63 NaHCO3 activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.63 56000
-
 x * 56000, SDS-PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.63 2-carboxy-D-arabinitol 1-phosphate + H2O Phaseolus vulgaris
-
 2-carboxy-D-arabinitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.63 Phaseolus vulgaris
-
 French bean, var. Tendergreen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.63 partial Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.63 leaf
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.63 1.71
-
-
 Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.1.3.63 -25°C, several months Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.63 2-carboxy-D-arabinitol 1,5-bisphosphate + H2O
-
 Phaseolus vulgaris 2-carboxy-D-arabinitol 5-phosphate + phosphate
-
 ?
3.1.3.63 2-carboxy-D-arabinitol 1-phosphate + H2O
-
 Phaseolus vulgaris 2-carboxy-D-arabinitol + phosphate
-
 ?
3.1.3.63 2-carboxy-D-ribitol 1,5-bisphosphate + H2O
-
 Phaseolus vulgaris ?
-
 ?
3.1.3.63 D-3-phosphoglycerate + H2O poor substrate Phaseolus vulgaris ?
-
 ?
3.1.3.63 diphosphate + H2O poor substrate Phaseolus vulgaris 2 phosphate
-
 ?
3.1.3.63 fructose 1,6-bisphosphate + H2O poor substrate Phaseolus vulgaris ?
-
 ?
3.1.3.63 additional information no substrates are D-glucose 1-phosphate, D-glucose 6-phosphate Phaseolus vulgaris ?
-
 ?
3.1.3.63 additional information no substrates are phosphoglycolic acid, D-fructose 6-phosphate, D-sedoheptulose 1,7-bisphosphate, NADP+, ATP, 5'-AMP Phaseolus vulgaris ?
-
 ?
3.1.3.63 additional information no substrates are D-6-phosphogluconate Phaseolus vulgaris ?
-
 ?
3.1.3.63 ribulose 1,5-bisphosphate + H2O
-
 Phaseolus vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.63 ? x * 56000, SDS-PAGE Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.63 25
-
 assay at Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.63 7
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.63 6 8.5 about 75% of maximal activity at pH 6 and about half-maximal activity at pH 8.5 Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.3.63 NADPH activation Phaseolus vulgaris
3.1.3.63 NADPH 2 mM Phaseolus vulgaris