Literature summary extracted from
Salvucci, M.E.; Holbrook, G.P.
Purification and properties of 2-carboxy-D-arabinitol 1-phosphatase (1989), Plant Physiol., 90, 679-685.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.3.63 |
3-phosphoglycerate |
activation |
Nicotiana tabacum |
|
3.1.3.63 |
D-fructose 1,6-bisphosphate |
activation |
Nicotiana tabacum |
|
3.1.3.63 |
additional information |
no activation by ATP |
Nicotiana tabacum |
|
3.1.3.63 |
additional information |
no activation by ADP, 6-phosphogluconate, D-fructose 6-phosphate, D-glucose 1-phosphate, D-ribulose 5-phosphate, D-ribose 5-phosphate |
Nicotiana tabacum |
|
3.1.3.63 |
Ribulose 1,5-bisphosphate |
activation |
Nicotiana tabacum |
|
General Stability
EC Number |
General Stability |
Organism |
---|
3.1.3.63 |
major loss of activity during ion-exchange chromatography |
Nicotiana tabacum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.3.63 |
ATP |
- |
Nicotiana tabacum |
|
3.1.3.63 |
Cu2+ |
5 mM |
Nicotiana tabacum |
|
3.1.3.63 |
MoO42- |
strong |
Nicotiana tabacum |
|
3.1.3.63 |
additional information |
no inhibition by K+, Mg2+, Na+, NH4+, EDTA or cysteine |
Nicotiana tabacum |
|
3.1.3.63 |
PO43- |
- |
Nicotiana tabacum |
|
3.1.3.63 |
ribose 5-phosphate |
weak |
Nicotiana tabacum |
|
3.1.3.63 |
SO42- |
inhibitory above 700 mM, activates at 10-50 mM |
Nicotiana tabacum |
|
3.1.3.63 |
Zn2+ |
5 mM |
Nicotiana tabacum |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.3.63 |
0.033 |
- |
2-carboxyarabinitol 1-phosphate |
25°C, pH 7.5 |
Nicotiana tabacum |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.1.3.63 |
chloroplast |
- |
Nicotiana tabacum |
9507 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.3.63 |
additional information |
no activation by Mg2+ |
Nicotiana tabacum |
|
3.1.3.63 |
additional information |
no activation by K+, Na+, NH4+ |
Nicotiana tabacum |
|
3.1.3.63 |
SO42- |
activation, 10-50 mM, inhibits above 700 mM |
Nicotiana tabacum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.3.63 |
53000 |
- |
x * 53000, SDS-PAGE |
Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.3.63 |
2-carboxy-D-arabinitol 1-phosphate + H2O |
Nicotiana tabacum |
- |
2-carboxy-D-arabinitol + phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.3.63 |
Nicotiana tabacum |
- |
tobacco, var. KY14 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.3.63 |
from leaf and from isolated chloroplasts |
Nicotiana tabacum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.3.63 |
leaf |
- |
Nicotiana tabacum |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.3.63 |
0.2612 |
- |
25°C, pH 7.5 |
Nicotiana tabacum |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.1.3.63 |
-20°C, slow loss of activity in the absence of ammonium sulfate |
Nicotiana tabacum |
3.1.3.63 |
4°C, stable as ammonium sulfate suspension, slow loss of activity in the absence of ammonium sulfate |
Nicotiana tabacum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.3.63 |
2-carboxy-D-arabinitol 1-phosphate + H2O |
- |
Nicotiana tabacum |
2-carboxy-D-arabinitol + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.3.63 |
? |
x * 53000, SDS-PAGE |
Nicotiana tabacum |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.3.63 |
25 |
- |
assay at |
Nicotiana tabacum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.3.63 |
7.5 |
- |
- |
Nicotiana tabacum |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.1.3.63 |
5.5 |
8.4 |
about 65% of maximal activity at pH 5.5 and about half-maximal activity at pH 8.4 |
Nicotiana tabacum |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
3.1.3.63 |
NADPH |
activation |
Nicotiana tabacum |
|