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Literature summary extracted from

  • Shinano, T.; Yonetani, R.; Ushihara, N.; Adachi, H.; Wasaki, J.; Matsui, H.; Osaki, M.
    Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa (2001), Plant Sci., 161, 861-869.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.60 F-
-
 Allium cepa
3.1.3.60 phosphate
-
 Allium cepa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.60 0.44
-
 phosphoenolpyruvate 25°C, pH 6.6 Allium cepa
3.1.3.60 1.36
-
 phytic acid 25°C, pH 6.6 Allium cepa
3.1.3.60 1.47
-
 D-glucose 6-phosphate 25°C, pH 6.6 Allium cepa
3.1.3.60 1.51
-
 ATP 25°C, pH 6.6 Allium cepa
3.1.3.60 1.6
-
 4-nitrophenyl phosphate 25°C, pH 6.6 Allium cepa
3.1.3.60 2.69
-
 ADP 25°C, pH 6.6 Allium cepa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.60 Mg2+ 1.7 fold activation at 5 mM Allium cepa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.60 52000
-
 SDS-PAGE, degradation product with 42000 Da Allium cepa
3.1.3.60 240000
-
 gel filtration Allium cepa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.60 phosphoenolpyruvate + H2O Allium cepa
-
 pyruvate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.60 Allium cepa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.60
-
 Allium cepa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.60 53
-
 purified enzyme, 25°C, pH 6.6 Allium cepa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.60 4-nitrophenyl phosphate + H2O
-
 Allium cepa 4-nitrophenol + phosphate
-
 ?
3.1.3.60 ADP + H2O
-
 Allium cepa ?
-
 ?
3.1.3.60 ATP + H2O
-
 Allium cepa ?
-
 ?
3.1.3.60 D-glucose 6-phosphate + H2O
-
 Allium cepa D-glucose + phosphate
-
 ?
3.1.3.60 phosphoenolpyruvate + H2O
-
 Allium cepa pyruvate + phosphate
-
 ?
3.1.3.60 phytic acid + H2O
-
 Allium cepa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.60 tetramer 4 * 52000, gel filtration, SDS-PAGE Allium cepa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.60 50
-
 stable up to 50°C Allium cepa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.60 7
-
-
 Allium cepa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.60 5.7 7.8 stable Allium cepa