Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duff, S.M.G.; Lefebvre, D.D.; Plaxton, W.C.
    Purification and characterization of a phosphoenolpyruvate phosphatase from Brassica nigra suspension cells (1989), Plant Physiol., 90, 734-741.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.60 3-phosphoglycerate competitive; complete inhibition at 5 mM Brassica nigra
3.1.3.60 aspartate competitive Brassica nigra
3.1.3.60 CuSO4 complete inhibition at 4 mM Brassica nigra
3.1.3.60 D-fructose 1,6-bisphosphate complete inhibition at 5 mM, competitive Brassica nigra
3.1.3.60 FeCl3 complete inhibition at 4 mM Brassica nigra
3.1.3.60 glutamate competitive Brassica nigra
3.1.3.60 glyceraldhyde-3-phosphate competitive Brassica nigra
3.1.3.60 Lead acetate 70% inhibition at 8 mM Brassica nigra
3.1.3.60 MgADP 41% inhibition at 0.5 mM, competitive Brassica nigra
3.1.3.60 molybdate complete inhibition at 0.1 mM, competitive Brassica nigra
3.1.3.60 additional information several more substances found to be inhibitory, no inhibition with N-ethylmaleimide Brassica nigra
3.1.3.60 NaF uncompetitive Brassica nigra
3.1.3.60 phosphate complete inhibition at 0.5 mM, competitive Brassica nigra
3.1.3.60 ZnCl2 complete inhibition at 4 mM Brassica nigra

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.60 0.05
-
 phosphoenolpyruvate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.24
-
 D-glucose 1-phosphate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.5
-
 ATP 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.5
-
 2,3 diphosphoglycerate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.57
-
 4-nitrophenyl phosphate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.59
-
 D-gluconate 6-phosphate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.74
-
 ADP 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.82
-
 phytic acid 25°C, pH 5.6 Brassica nigra
3.1.3.60 1.21
-
 3-phosphoglycerate 25°C, pH 5.6 Brassica nigra
3.1.3.60 2.01
-
 D-glucose 6-phosphate 25°C, pH 5.6 Brassica nigra

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.60 Ca2+ 1.4 fold activation at 4 mM Brassica nigra
3.1.3.60 Co2+ 1.4 fold activation at 4 mM Brassica nigra
3.1.3.60 Mg2+ 1.7 fold activation at 4 mM Brassica nigra
3.1.3.60 Mn2+ 1.4 fold activation at 4 mM Brassica nigra

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.60 55000
-
 SDS-PAGE Brassica nigra
3.1.3.60 56000
-
 gel filtration Brassica nigra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.60 phosphoenolpyruvate + H2O Brassica nigra bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation pyruvate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.60 Brassica nigra
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.60
-
 Brassica nigra

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.60 leaf petiole suspension cells Brassica nigra
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.60 additional information
-
 specific activity determined for all substrates Brassica nigra
3.1.3.60 380
-
 purified enzyme, 25°C, pH 5.6 Brassica nigra

Storage Stability

EC Number Storage Stability Organism
3.1.3.60 -80°C, purified enzyme, 6 months, stable Brassica nigra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.60 1-naphthyl-phosphate + H2O
-
 Brassica nigra 1-naphthol + phosphate
-
 ?
3.1.3.60 2,3-diphosphoglycerate + H2O
-
 Brassica nigra ?
-
 ?
3.1.3.60 2-phosphoglycerate + H2O poor substrate Brassica nigra glycerate + phosphate
-
 ?
3.1.3.60 3-phosphoglycerate + H2O
-
 Brassica nigra glycerate + phosphate
-
 ?
3.1.3.60 4-nitrophenyl phosphate + H2O
-
 Brassica nigra 4-nitrophenol + phosphate
-
 ?
3.1.3.60 ADP + H2O
-
 Brassica nigra ?
-
 ?
3.1.3.60 ADP-glucose + H2O poor substrate Brassica nigra ?
-
 ?
3.1.3.60 ATP + H2O
-
 Brassica nigra ?
-
 ?
3.1.3.60 D-fructose 2,6-diphosphate + H2O poor substrate Brassica nigra ?
-
 ?
3.1.3.60 D-gluconate 6-phosphate + H2O
-
 Brassica nigra D-gluconate + phosphate
-
 ?
3.1.3.60 D-glucose 1-phosphate + H2O
-
 Brassica nigra ?
-
 ?
3.1.3.60 D-glucose 6-phosphate + H2O poor substrate Brassica nigra D-glucose + phosphate
-
 ?
3.1.3.60 D-ribose 5-phosphate + H2O poor substrate Brassica nigra D-ribose + phosphate
-
 ?
3.1.3.60 O-phospho-L-threonine + H2O
-
 Brassica nigra L-threonine + phosphate
-
 ?
3.1.3.60 O-phospho-L-tyrosine + H2O
-
 Brassica nigra L-tyrosine + phosphate
-
 ?
3.1.3.60 phosphoenolpyruvate + H2O
-
 Brassica nigra pyruvate + phosphate
-
 ?
3.1.3.60 phosphoenolpyruvate + H2O bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation Brassica nigra pyruvate + phosphate
-
 ?
3.1.3.60 phytic acid + H2O
-
 Brassica nigra ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.60 monomer 1 * 56000, gel filtration, SDS-PAGE Brassica nigra

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.60 50
-
 4 min, stable Brassica nigra
3.1.3.60 55
-
 4 min, 20% loss of activity Brassica nigra
3.1.3.60 60
-
 4 min, 30% loss of activity Brassica nigra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.60 5.6
-
-
 Brassica nigra

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.60 3 7.5
-
 Brassica nigra

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.60 0.003
-
 molybdate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.05
-
 3-phosphoglycerate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.06
-
 D-fructose 1,6-bisphosphate 25°C, pH 5.6 Brassica nigra
3.1.3.60 0.085
-
 phosphate 25°C, pH 5.6 Brassica nigra